Jacobo-Cuevas Heriberto, Gamez-Nava Jorge Ivan, Ramírez-De Los Santos Saúl, Mercado-Calderón Carlos Alfonso, Ríos-González Blanca Estela, Ponce-Guarneros Juan Manuel, Brambila-Tapia Aniel Jessica Leticia
Group for the Assessment of Prognosis Biomarkers in Autoimmune Disorders, Centro Universitario de Ciencias de la Salud (CUCS), Universidad de Guadalajara, Guadalajara 44340, Mexico.
Programa de Postdoctorado, Departamento de Psicología Básica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara 44340, Mexico.
Biomolecules. 2025 Sep 2;15(9):1271. doi: 10.3390/biom15091271.
Global DNA methylation has been associated with numerous traits and conditions; however, its relationship with inflammation and oxidative stress biomarkers has not been fully elucidated. The objective of this study is to determine the correlation between inflammatory and oxidative stress markers with global DNA methylation after adjusting for personal, psychological, biochemical, anthropometric, and physiological variables in a non-representative sample of the Mexican population. An adult Mexican population was invited to participate and complete a questionnaire with personal and psychological variables. Additionally, anthropometric variables and blood pressure were measured in all the participants. Finally, general blood tests, global DNA methylation analysis, and measurements of inflammatory and oxidative stress markers were performed. A total of 157 participants were included, of which 83 (52.8%) were women, with a median age of 24 years and an age range of 18-58 years. In the comparison between sexes, men showed higher levels of global DNA methylation. In addition, men showed a higher number of correlations with this variable. The bivariate correlations showed low positive correlations of IL-8, IL-10, TNF-α, and 8-isoprostane with global DNA methylation in the total sample. In addition, BMI showed low negative and significant correlations with global DNA methylation in the total, women's, and men's samples, while blood pressure showed low negative correlations with global DNA methylation in the men's sample. Men showed low negative correlations with personal and biochemical variables that were not found in the women's group. In the multivariate analyses, the psychological variables (SOC-13 comprehensibility, perceived stress, and assertiveness) correlated negatively either in the total, or in men's or women's samples, and the daily intake of drugs correlated negatively with methylation in the women's sample in the bivariate and multivariate analyses. In conclusion, global DNA methylation seems to be related to many variables, including the inflammatory and oxidative stress biomarkers, and this relationship is different in each sex.
全基因组DNA甲基化与众多性状和疾病状况相关；然而，其与炎症和氧化应激生物标志物之间的关系尚未完全阐明。本研究的目的是在对墨西哥人群的一个非代表性样本中的个人、心理、生化、人体测量和生理变量进行校正后，确定炎症和氧化应激标志物与全基因组DNA甲基化之间的相关性。邀请了成年墨西哥人群参与并完成一份包含个人和心理变量的问卷。此外，对所有参与者测量了人体测量变量和血压。最后，进行了常规血液检查、全基因组DNA甲基化分析以及炎症和氧化应激标志物的测量。总共纳入了157名参与者，其中83名（52.8%）为女性，中位年龄为24岁，年龄范围为18 - 58岁。在性别比较中，男性的全基因组DNA甲基化水平较高。此外，男性与该变量的相关性数量更多。双变量相关性显示，在总样本中，IL - 8、IL - 10、TNF -α和8 -异前列腺素与全基因组DNA甲基化呈低正相关。此外，在总样本、女性样本和男性样本中，BMI与全基因组DNA甲基化呈低负相关且具有统计学意义，而在男性样本中，血压与全基因组DNA甲基化呈低负相关。男性与女性组未发现的个人和生化变量呈低负相关。在多变量分析中，心理变量（SOC - 13可理解性、感知压力和自信）在总样本、男性样本或女性样本中均呈负相关，并且在双变量和多变量分析中，女性样本中的药物每日摄入量与甲基化呈负相关。总之，全基因组DNA甲基化似乎与许多变量相关，包括炎症和氧化应激生物标志物，并且这种关系在不同性别中有所不同。
