Colangelo Maria Teresa, Belletti Silvana, Guizzardi Stefano, Galli Carlo
Histology and Embryology Laboratory, Department of Medicine and Surgery, University of Parma, Via Volturno 39, 43126 Parma, Italy.
Antioxidants (Basel). 2025 Sep 5;14(9):1089. doi: 10.3390/antiox14091089.
This study investigates the scavenger activity of Polynucleotide High Purification Technology (PN HPT), alone or in combination with hyaluronic acid (PN HPT + HA) against oxidative stress induced by hydrogen peroxide (HO). Since oxidative stress is implicated in numerous pathological conditions, identifying effective antioxidants is crucial for therapeutic development. We employed a cell-free fluorometric assay based on Calcein-AM, a fluorescence probe whose signal increases proportionally to the generation of reactive oxygen species (ROS), to evaluate the ability to neutralize ROS under varying oxidative stress conditions and determine the dose- and time-dependent effects of these compounds. PN HPT, HA, and PN HPT + HA were tested at various concentrations over multiple time points. Our results demonstrated that all tested treatments significantly lowered ROS levels compared to the untreated control. Notably, the PN HPT -based compounds exhibited robust scavenging activity, with PN HPT + HA displaying the strongest and most consistent ROS-neutralizing effect across all concentrations and time points. This enhanced performance suggests a synergistic interaction between PN HPT and HA, potentially due to complementary mechanisms of free radical scavenging and structural stabilization. These findings highlight the potential of PN HPT and PN HPT + HA as effective antioxidative agents, offering potential for therapeutic applications where oxidative stress is central, including wound healing and tissue regeneration.
本研究调查了多核苷酸高纯化技术（PN HPT）单独或与透明质酸联合使用（PN HPT + HA）对过氧化氢（HO）诱导的氧化应激的清除活性。由于氧化应激与多种病理状况有关，因此鉴定有效的抗氧化剂对于治疗开发至关重要。我们采用了基于钙黄绿素-AM的无细胞荧光测定法，钙黄绿素-AM是一种荧光探针，其信号与活性氧（ROS）的产生成比例增加，以评估在不同氧化应激条件下中和ROS的能力，并确定这些化合物的剂量和时间依赖性效应。在多个时间点对不同浓度的PN HPT、HA和PN HPT + HA进行了测试。我们的结果表明，与未处理的对照相比，所有测试处理均显著降低了ROS水平。值得注意的是，基于PN HPT的化合物表现出强大的清除活性，PN HPT + HA在所有浓度和时间点均表现出最强且最一致的ROS中和效果。这种增强的性能表明PN HPT和HA之间存在协同相互作用，这可能是由于自由基清除和结构稳定的互补机制所致。这些发现突出了PN HPT和PN HPT + HA作为有效抗氧化剂的潜力，为氧化应激起核心作用的治疗应用提供了潜力，包括伤口愈合和组织再生。
