DeSana Anthony J, Alfawares Yara, Khatri Roshni, Hopkins Tracy M, Best Faith V, McGuire Jennifer L, Ngwenya Laura B
Department of Neurosurgery, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA.
Department of Neurology and Rehabilitation Medicine, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA.
Int J Mol Sci. 2025 Sep 19;26(18):9140. doi: 10.3390/ijms26189140.
Traumatic brain injury (TBI) is a major source of disability worldwide, with cognitive and memory deficits being pervasive after injury. The hippocampus, a major structure involved in learning and memory, is particularly vulnerable to TBI, and cellular dysfunction within the hippocampal dentate gyrus is believed to be a major contributor to cognitive deficits after TBI. However, there is little known about the transcriptomic changes occurring directly within the dentate gyrus at subacute-to-chronic timepoints after TBI. To address this, we performed bulk RNA sequencing and single-nucleus RNA sequencing of the isolated dentate gyrus three weeks after lateral fluid percussion injury in male rats. We report here that there is evidence of an ongoing neuroinflammatory response marked by increased neuroinflammatory genes that implicate various neuroinflammatory pathways that are associated with a subset of microglia and astrocyte populations.
创伤性脑损伤（TBI）是全球残疾的主要原因，受伤后认知和记忆缺陷普遍存在。海马体是参与学习和记忆的主要结构，特别容易受到TBI的影响，海马齿状回内的细胞功能障碍被认为是TBI后认知缺陷的主要原因。然而，对于TBI后亚急性至慢性时间点齿状回内直接发生的转录组变化知之甚少。为了解决这个问题，我们对雄性大鼠侧方流体冲击伤三周后的分离齿状回进行了批量RNA测序和单核RNA测序。我们在此报告，有证据表明存在持续的神经炎症反应，其特征是神经炎症基因增加，这些基因涉及与小胶质细胞和星形胶质细胞群体的一个子集相关的各种神经炎症途径。
