Insignares Samuel, Kuchtey John, Kuchtey Rachel W
Vanderbilt Eye Institute, Vanderbilt University Medical Center, 2311 Pierce Avenue, Nashville, TN 37232, USA.
Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232, USA.
Int J Mol Sci. 2025 Sep 21;26(18):9221. doi: 10.3390/ijms26189221.
We previously reported ocular phenotypes of 1-year-old 129S1/SvlmJ lysyl oxidase-like 1 null () mice. Here we sought to characterize age-dependent changes in C57BL/6J mice in a longitudinal fashion. Retinal ganglion cell (RGC) function was assessed by electroretinography (ERG), and optic nerves were evaluated by histological analysis. Ocular biometric measurements were obtained by optical coherence tomography (OCT). We detected reduced RGC function, revealed by decreased amplitude and increased latency of ERG positive scotopic threshold responses (pSTRs) in mice compared to age-matched wt mice. In addition, there is significant inter-eye asymmetry of RGC function, as well as age-related RGC function loss observed only in mice. Histologically, we observed enlarged optic nerve areas in mice compared to wt mice. Significant ocular biometric differences between two groups were detected, most notably, age-related axial elongation of the globe, accompanied by deepening of anterior chamber depth (ACD). Though eyes elongate with age in both groups, this is more pronounced in mice, and the elongation of the globe correlated with decreased RGC function. The correlation of age-related reduction in RGC function with globe axial elongation may have implications for the association of axial myopia with glaucoma and aging in humans.
我们之前报道过129S1/SvlmJ赖氨酸氧化酶样1基因敲除（）小鼠1岁时的眼部表型。在此，我们试图以纵向方式描述C57BL/6J 小鼠的年龄依赖性变化。通过视网膜电图（ERG）评估视网膜神经节细胞（RGC）功能，并通过组织学分析评估视神经。通过光学相干断层扫描（OCT）获得眼部生物测量数据。与年龄匹配的野生型小鼠相比，我们在 小鼠中检测到RGC功能降低，表现为ERG阳性暗视阈值反应（pSTRs）的振幅降低和潜伏期延长。此外，RGC功能存在明显的两眼不对称性，并且仅在 小鼠中观察到与年龄相关的RGC功能丧失。组织学上，与野生型小鼠相比，我们在 小鼠中观察到视神经区域增大。检测到两组之间存在显著的眼部生物测量差异，最明显的是与年龄相关的眼球轴长延长，同时伴有前房深度（ACD）加深。尽管两组小鼠的眼睛都会随着年龄增长而变长，但在 小鼠中更为明显，并且眼球延长与RGC功能降低相关。RGC功能与年龄相关的降低与眼球轴长延长之间的相关性可能对人类轴向近视与青光眼和衰老的关联具有启示意义。
