Yan Fen, Duan Deyong, Meng Jinzhu, Cheng Tianyin
Research Center for Parasites & Vectors (RCPV), College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China.
Guizhou Provincial Key Laboratory for Biodiversity Conservation and Utilization in the Fanjing Mountain Region, Tongren University, Tongren 554300, China.
Genes (Basel). 2025 Sep 19;16(9):1107. doi: 10.3390/genes16091107.
: is a hematophagous ectoparasite of poultry and is the vector of several agents infectious to poultry. This study aims to explore the key genes affecting the ovarian development of . : RNA-seq was performed on the ovaries of before blood-feeding, on the day of engorgement, and 6 days post-engorgement. Utilizing the threshold padj < 0.05 and|log(foldchange)| > 1, differentially expressed genes were identified, and hub genes were determined by constructing protein-protein interaction (PPI) networks. : A total of 1008 differentially expressed genes were obtained during the feeding period, including 448 up-regulated and 560 down-regulated genes. Further, 2179 differentially expressed genes were screened in the preoviposition stage, including 1957 up-regulated and 222 down-regulated genes. These genes are mainly annotated in functions such as peptidase activity (especially serine protease activity), protein folding, protein assembly, and cell component assembly, and enriched in pathways such as protein processing in endoplasmic reticulum, lysosome, glutathione metabolism, and sphingolipid metabolism. In addition, some proteins that are closely related to ovarian development, including heat shock protein 70, protein disulfide isomerase, paramyosin, troponin I, hexosaminidase, serine protease, Kunitz serine protease inhibitors, and vitellogenin, were obtained. : These findings fill the gap in the biological data for the ovarian development of soft ticks, provide a reference database for subsequent proteomics research, and offer fundamental support for the screening and development of candidate antigens for anti-tick vaccines.
：是家禽的吸血外寄生虫，也是几种家禽传染性病原体的传播媒介。本研究旨在探索影响卵巢发育的关键基因。：对饱血前、饱血当天和饱血后6天的卵巢进行RNA测序。利用阈值padj < 0.05和|log(倍数变化)| > 1鉴定差异表达基因，并通过构建蛋白质-蛋白质相互作用（PPI）网络确定枢纽基因。：在取食期共获得1008个差异表达基因，其中上调基因448个，下调基因560个。此外，在产卵前期筛选出2179个差异表达基因，其中上调基因1957个，下调基因222个。这些基因主要注释在肽酶活性（尤其是丝氨酸蛋白酶活性）、蛋白质折叠、蛋白质组装和细胞成分组装等功能中，并富集在内质网中的蛋白质加工、溶酶体、谷胱甘肽代谢和鞘脂代谢等途径中。此外，还获得了一些与卵巢发育密切相关的蛋白质，包括热休克蛋白70、蛋白质二硫键异构酶、副肌球蛋白、肌钙蛋白I、己糖胺酶、丝氨酸蛋白酶、库尼茨丝氨酸蛋白酶抑制剂和卵黄原蛋白。：这些发现填补了软蜱卵巢发育生物学数据的空白，为后续蛋白质组学研究提供了参考数据库，并为抗蜱疫苗候选抗原的筛选和开发提供了基础支持。
