Optimization of Microbial Consortium Formulation for Oily Food Waste Composting Using Mixture Design Methodology.

The efficient compounding of microbial agents for use in aerobic composting processes is a pressing problem that needs to be addressed. This work focused on the lack of effective oil-degrading microorganisms and the challenges in formulating microbial consortia during the composting of food waste (FW). Following the isolation of three bacteria and three fungi with high oil-degrading ability, a simplex-lattice mixture design methodology was used to conduct compounding within and between groups of bacteria and fungi. Three special cubic response models were successfully developed and validated by performing an analysis of variance. From our analysis, it was demonstrated that the three models had high R values of 96.06%, 97.18%, and 96.27%. The global solution of the mixture optimization predicted the optimal value for a blend comprising 11.83% , 8.10% , 10.97% , 20.9% , 22.3% , and 25.9% Thus, these proportions were considered the optimal combination of strains for oil degradation during FW composting. Composting verification in a 60 L fermenter revealed that the composite microbial agent group had a 31.3% higher oil degradation efficiency than the control group. This work provides valuable insights for the compounding of microbial agents and the resource utilization of rural FW.