College of Environmental & Resource Sciences, Zhejiang University, Hangzhou, 310058, China.
College of Environmental & Resource Sciences, Zhejiang University, Hangzhou, 310058, China; Key Laboratory for Water Pollution Control and Environmental Safety, Zhejiang, 310058, China; Key Laboratory of Environment Remediation and Ecological Health, Ministry of Education, College of Environmental Resource Sciences, Zhejiang University, Hangzhou, 310058, China.
Chemosphere. 2023 May;322:138191. doi: 10.1016/j.chemosphere.2023.138191. Epub 2023 Feb 20.
Inoculation can effectively improve the recycling level of organic waste in composting process. However, the role of inocula in the humification process has been rarely studied. Therefore, we constructed a simulated food waste composting system by adding commercial microbial agents to explore the function of inocula. The results showed that adding microbial agents extended the high temperature maintenance time by 33% and increased the humic acid content by 42%. Inoculation significantly improved the degree of directional humification (HA/TOC = 0.46, p < 0.001). The proportion of positive cohesion in the microbial community underwent an overall increase. The strength of bacterial/fungal community interaction increased by 1.27-fold after inoculation. Furthermore, the inoculum stimulated the potential functional microbes (Thermobifida and Acremonium) which were highly related to the formation of humic acid and the degradation of organic matter. This study showed that additional microbial agents could strengthen microbial interaction to raise the humic acid content, thus opening the door for the development of targeted biotransformation inocula in the future.
接种可以有效地提高堆肥过程中有机废物的再循环水平。然而，接种物在腐殖化过程中的作用很少被研究。因此，我们通过添加商业微生物制剂构建了一个模拟的食物垃圾堆肥系统，以探索接种物的功能。结果表明，添加微生物制剂将高温维持时间延长了 33%，并将腐殖酸含量提高了 42%。接种显著提高了定向腐殖化程度（HA/TOC=0.46，p<0.001）。微生物群落中阳性内聚体的比例整体增加。接种后，细菌/真菌群落相互作用的强度增加了 1.27 倍。此外，接种物刺激了与腐殖酸形成和有机物降解高度相关的潜在功能微生物（Thermobifida 和 Acremonium）。本研究表明，额外的微生物制剂可以加强微生物相互作用，提高腐殖酸含量，从而为未来有针对性的生物转化接种剂的开发开辟了道路。
