Hu Jing, Zhou Jiahe, Wang Leping, Chen Zhongwei, Tan Yizhou, Yin Yangyan, Pei Zhe, Li Changting, Bai Huili, Ma Chunxia, Teng Ling, Feng Yongcui, Li Xian, Wei Yingyi, Peng Hao
College of Animal Science and Technology, Guangxi University, Nanning 530004, China.
Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530001, China.
Vet Sci. 2025 Aug 22;12(9):793. doi: 10.3390/vetsci12090793.
An outbreak characterized by clinical signs of diarrhea and paralysis, occasionally progressing to fatal outcomes, occurred at an ostrich breeding facility. Conventional antibiotic treatments proved ineffective. To investigate the etiology of the disease, brain and liver specimens were collected for diagnostic analysis. An () isolate, designated strain HZDC01, was obtained from cerebral tissues, and whole-genome sequencing was performed for genomic characterization. Genomic analysis revealed that the chromosomal DNA harbors numerous resistance genes, conferring multidrug resistance through complex mechanisms. Furthermore, a p0111-type plasmid carrying the gene and an IncX1-type plasmid harboring , , , , , , , and genes were identified. These plasmids carry numerous mobile genetic elements that can disseminate via horizontal gene transfer, thereby amplifying the risk of resistance-gene spread within bacterial populations. Additionally, the and genes, which encode proteins involved in the invasion of brain microvascular endothelial cells, were identified. These genes may facilitate penetration of the blood-brain barrier, potentially leading to meningitis and posing a life-threatening risk to the host. This is the first report of the isolation and characterization of extended-spectrum beta-lactamase from the brain of an ostrich with paralysis. The findings provide valuable genomic insights into the antimicrobial resistance profiles and pathogenic mechanisms of ostrich-derived isolates.
在一个鸵鸟养殖设施中发生了一场以腹泻和麻痹为临床症状、偶尔发展为致命后果的疫情。传统抗生素治疗证明无效。为调查该疾病的病因，采集了脑和肝标本进行诊断分析。从脑组织中获得了一株（）分离株，命名为HZDC01菌株，并进行了全基因组测序以进行基因组特征分析。基因组分析表明，染色体DNA含有众多耐药基因，通过复杂机制赋予多重耐药性。此外，还鉴定出一个携带（）基因的p0111型质粒和一个携带（）、（）、（）、（）、（）、（）、（）和（）基因的IncX1型质粒。这些质粒携带众多可通过水平基因转移传播的移动遗传元件，从而增加了耐药基因在细菌群体中传播的风险。此外，还鉴定出了编码参与脑微血管内皮细胞侵袭的蛋白质的（）和（）基因。这些基因可能有助于（）突破血脑屏障，潜在地导致脑膜炎并对宿主构成生命威胁。这是首次从患有麻痹的鸵鸟脑中分离和鉴定出超广谱β-内酰胺酶（）的报告。这些发现为鸵鸟源（）分离株的抗菌耐药谱和致病机制提供了有价值的基因组见解。
