Wu Weifan, Mis Solval Kevin, Chen Jinru
Department of Food Science and Technology, The University of Georgia, Griffin, GA, 30223-1797, USA.
Heliyon. 2024 Apr 16;10(8):e29700. doi: 10.1016/j.heliyon.2024.e29700. eCollection 2024 Apr 30.
This study compared the efficacy of aqueous extracts of commercially available pomegranate peel products and a juice powder in inhibiting the growth of two enterohemorrhagic strains. Cell suspension of each strain (5 Log CFU/ml) was added into tryptic soy broth amended with 9 or 23% of each extract prepared with two different methods. After treatment for 5, 10, and 24 h at 25 °C, surviving cells were enumerated on tryptic soy agar to determine cell population reduction compared to the controls. The concentrations of six different ellagitannins and titratable activity in each treatment system were determined and correlated to cell population reduction. The extracts from three powdered pomegranate peels caused a significantly greater ( ≤ 0.05) reduction in population than the extract from the whole peel and juice powder. The higher dose of extracts resulted in a greater cell population reduction than the lower dose. The level of population reduction correlated positively with the total ellagitannins content (R 0.67-0.98) and the titratable acidity (R 0.69-0.98) in the treatment systems. The study suggests that pomegranate peels are promising natural additives or preservatives to control pathogens like EHEC.
本研究比较了市售石榴皮产品的水提取物和一种果汁粉对两种肠出血性菌株生长的抑制效果。将每种菌株的细胞悬液（5 Log CFU/ml）加入到用两种不同方法制备的含9%或23%每种提取物的胰蛋白胨大豆肉汤中。在25℃处理5、10和24小时后，在胰蛋白胨大豆琼脂上对存活细胞进行计数，以确定与对照相比细胞数量的减少情况。测定了每个处理系统中六种不同鞣花单宁的浓度和可滴定活性，并将其与细胞数量的减少相关联。三种石榴皮粉末提取物导致的细胞数量减少显著大于（≤0.05）整个石榴皮提取物和果汁粉。较高剂量的提取物比较低剂量导致更大的细胞数量减少。处理系统中细胞数量的减少水平与总鞣花单宁含量（R = 0.67 - 0.
