Zarei Ali, Gholami Ahmad, Kanannejad Zahra, Berenjian Aydin, Nabavizadeh Seyed Hesamodin, Mohkam Milad
Department of Physiology, Estahban School of Paramedical Sciences, School of Nursing Hazrat Zahra (P.B.U.H) Abadeh, Shiraz University of Medical Sciences Shiraz Iran.
Biotechnology Research Center Shiraz University of Medical Sciences Shiraz Iran.
Food Sci Nutr. 2025 Sep 26;13(10):e71031. doi: 10.1002/fsn3.71031. eCollection 2025 Oct.
spp. probiotics have been shown to be promising due to their resilience in the gut. This study evaluated the in vitro probiotic potential of PTCC 1596, using ATCC 700160 as a control. In vitro probiotic characteristic assays were conducted to compare and (control) in terms of acid and bile tolerance, enzymatic activity, antimicrobial potential, antibiotic susceptibility, biofilm formation, hydrophobicity, autoaggregation, enterotoxin gene presence, and cytotoxicity. spores demonstrated high survival (95.6% ± 0.9%) at pH 2.5 for 3 h and tolerance to 0.5% oxgall bile salts. The strain produced catalase, protease, and nattokinase, and exhibited moderate cell surface hydrophobicity (47.1% ± 3.12% to xylene) and autoaggregation (7.0% ± 1.2% after 1 h). It showed sensitivity to several antibiotics (e.g., chloramphenicol, gentamicin) and lacked detectable plasmids and tested enterotoxin genes (//, /, , and ). Cytotoxicity assays using HepG-2 cells indicated non-toxicity, with supernatants potentially enhancing cell viability. While exhibiting lower biofilm formation than , displayed promising traits. These findings suggest PTCC 1596 is a potential probiotic candidate warranting further investigation, particularly given its alkaliphilic nature.
由于其在肠道中的耐受性，某些益生菌已显示出具有潜力。本研究以ATCC 700160作为对照，评估了PTCC 1596的体外益生菌潜力。进行了体外益生菌特性分析，以比较PTCC 1596和对照在耐酸性、耐胆汁性、酶活性、抗菌潜力、抗生素敏感性、生物膜形成、疏水性、自聚集性、肠毒素基因存在情况以及细胞毒性方面的差异。孢子在pH 2.5条件下3小时显示出高存活率（95.6%±0.9%），并且对0.5%牛磺胆酸盐具有耐受性。该菌株产生过氧化氢酶、蛋白酶和纳豆激酶，并表现出中等的细胞表面疏水性（对二甲苯为47.1%±3.12%）和自聚集性（1小时后为7.0%±1.2%）。它对几种抗生素（如氯霉素、庆大霉素）敏感，并且未检测到质粒和测试的肠毒素基因（//、/、和）。使用HepG - 2细胞进行的细胞毒性分析表明无毒性，其培养上清液可能会提高细胞活力。虽然PTCC 1596的生物膜形成能力低于对照，但显示出有前景的特性。这些发现表明PTCC 1596是一个潜在的益生菌候选菌株，值得进一步研究，特别是考虑到其嗜碱性质。
