Department of Biotechnology, Faculty of Biological Sciences and Technology, Shahid Ashrafi Esfahani University, Isfahan, Iran.
Department of Microbiology, Kish International Branch of Islamic Azad University, Kish, Iran.
Biomed Res Int. 2024 Oct 15;2024:2978083. doi: 10.1155/2024/2978083. eCollection 2024.
The escalating incidence of hospital infections due to antibiotic resistance necessitates the identification of alternative therapeutic agents such as probiotics. This study was designed to isolate and evaluate the efficacy of probiotics against , a prevalent etiological agent of urinary tract infections (UTIs). A total of 100 strains were isolated from clinical samples and subjected to antibiotic susceptibility testing via the disc diffusion method. Concurrently, probiotic bacteria were isolated from Bulgarian cheese and shallot yogurt, and their antibacterial activity against strains was assessed. The inhibitory potential of probiotic supernatants was evaluated using microtiter plate assays, with the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) determined at a 1/2 dilution. Cytotoxicity was evaluated using the MTT assay, and high-performance liquid chromatography (HPLC) was employed to analyze the concentrations of organic acids produced by the probiotics. The results revealed that all strains were resistant to tetracycline and doxycycline but susceptible to other antibiotics. strains M and B demonstrated notable antibacterial and antibiofilm activity against isolates. These probiotics exhibited susceptibility to most antibiotics and lacked virulence factors, suggesting their safety for therapeutic use. The organic acids produced by the probiotics were identified as lactic acid, acetic acid, and formic acid. In conclusion, strains M and B exhibit potent antimicrobial properties against , indicating their potential as therapeutic agents for UTIs. Further research is warranted to validate these findings and explore the possibility of these probiotics in clinical applications.
由于抗生素耐药性导致医院感染的发生率不断上升，因此需要寻找替代治疗药物，如益生菌。本研究旨在分离并评估益生菌对 ，一种常见的尿路感染（UTI）病因的疗效。从临床样本中分离出 100 株 菌株，并通过圆盘扩散法进行抗生素敏感性测试。同时，从保加利亚奶酪和葱酸奶中分离出益生菌，并评估其对 菌株的抗菌活性。使用微量滴定板测定法评估益生菌上清液的抑制潜力，以 1/2 稀释度确定最小抑菌浓度（MIC）和最小杀菌浓度（MBC）。使用 MTT 测定法评估细胞毒性，并采用高效液相色谱（HPLC）分析益生菌产生的有机酸浓度。结果表明，所有 菌株均对四环素和强力霉素耐药，但对其他抗生素敏感。 菌株 M 和 B 对 分离株表现出显著的抗菌和抗生物膜活性。这些益生菌对大多数抗生素敏感且缺乏毒力因子，提示它们可安全用于治疗。益生菌产生的有机酸被鉴定为乳酸、乙酸和甲酸。总之， 菌株 M 和 B 对 表现出强大的抗菌特性，表明它们有可能成为治疗尿路感染的药物。需要进一步的研究来验证这些发现，并探索这些益生菌在临床应用中的可能性。
