Stuart K D
J Cell Biol. 1971 Apr;49(1):189-95. doi: 10.1083/jcb.49.1.189.
A pleomorphic dyskinetoplastic strain of Trypanosoma brucei was produced by repeated acriflavine treatment. No kinetoplastic cells reappeared after 2 yr of maintenance in the absence of acriflavine. These dyskinetoplastic cells retained and therefore replicated the central element of the kinetoplast. This element was present in the "condensed" state typical of acriflavine-treated cells rather than the normal fibrillar state. Whole-cell DNA extracted from both normal and dyskinetoplastic strains revealed three bands upon isopycnic sedimentation, and there was no detectable alteration in buoyant density of any of these DNA components in the dyskinetoplastic strain. It seems likely that the dyskinetoplastic strain has retained its kinetoplast DNA but in an altered state.
通过反复用吖啶黄处理产生了布氏锥虫的多形运动不能性动基体菌株。在没有吖啶黄的情况下维持2年后，未再出现有动基体的细胞。这些运动不能性动基体细胞保留并因此复制了动基体的中央元件。该元件以吖啶黄处理细胞典型的“浓缩”状态存在，而不是正常的纤维状状态。从正常菌株和运动不能性动基体菌株中提取的全细胞DNA在等密度沉降时显示出三条带，并且在运动不能性动基体菌株中这些DNA组分的任何一个的浮力密度都没有可检测到的改变。运动不能性动基体菌株似乎保留了其动基体DNA，但处于改变的状态。
