Hall H, Dubois M P
Acta Endocrinol (Copenh). 1977 Oct;86(2):273-87. doi: 10.1530/acta.0.0860273.
A biologically active peptide (called sperm-releasing substance, abbreviated SRS) with a molecular weight of about 5000, prepared from bovine adenohypophyses, has been compared with the known adenohypophyseal glycoprotein hormones using immunological methods. By means of immunofluorescence it can be seen that antisera against SRS have an affinity for the gonadotrophic cells in the anterior pituitary. Using complement fixation assay and immunodiffusion, no similarity between SRS and FSH can be found, while there are great resemblances between the reactions of LH and those of SRS. From the radioimmunoassay studies, it can be seen that the alpha-subunit of LH reacts in the same way as SRS in the different systems. However, using immunofluorescence, the antiserum against SRS has an affinity for the gonadotrophic cells only, like anti-LHbeta, and not for the thyrotrophic cells, as has anti-LHalpha. The reasons for these different results are discussed.
从牛垂体前叶制备出一种分子量约为5000的生物活性肽（称为精子释放物质，缩写为SRS），并使用免疫学方法将其与已知的垂体前叶糖蛋白激素进行了比较。通过免疫荧光可以看出，抗SRS血清对垂体前叶的促性腺细胞具有亲和力。使用补体结合试验和免疫扩散法，未发现SRS与FSH之间存在相似性，而LH与SRS的反应之间存在很大的相似性。从放射免疫分析研究中可以看出，LH的α亚基在不同系统中的反应方式与SRS相同。然而，使用免疫荧光法时，抗SRS血清仅对促性腺细胞具有亲和力，与抗LHβ血清一样，而不像抗LHα血清那样对促甲状腺细胞具有亲和力。讨论了这些不同结果的原因。
