Olsson L B, Kutti J, Weinfeld A
Acta Haematol. 1977;58(1):3-9. doi: 10.1159/000207799.
Duplicate platelet survival studies, using autologous platelets labelled in vitro with radioactive sodium chromate, were carried out on 5 lymphoma patients who had been splenectomized 14-21 months earlier. In the first experiment plasma was employed as the incubation medium and in the second a Ringer-citrate-dextrose (RCD) solution. The uptake of chromate by the platelets was 2.0 times higher in the RCD as compared to the plasma experiments. An identical pattern for the immediate behaviour of infused labelled platelets was observed in the duplicate studies, and the recovery of platelet-bound radioactivity remained stable at the 90% level during 2 h after infusion. In these experiments the means for platelet mean life span were almost identical, 5.4+/-0.6 and 5.3+/-0.5 days, respectively, and significantly (p less than 0.05) shorter than the mean for a control group consisting of 21 healthy males (6.9+/-0.3 days). It is concluded that RCD and plasma seem to serve equally well as incubation medium at the in vitro labelling of platelets.
对5例14 - 21个月前已行脾切除术的淋巴瘤患者进行了重复的血小板存活研究，采用体外以放射性铬酸钠标记的自体血小板。在第一个实验中，使用血浆作为孵育介质，在第二个实验中，使用林格氏枸橼酸盐葡萄糖（RCD）溶液。与血浆实验相比，血小板对铬酸盐的摄取在RCD中高2.0倍。在重复研究中观察到输注的标记血小板的即时行为模式相同，输注后2小时内血小板结合放射性的回收率稳定在90%水平。在这些实验中，血小板平均寿命的均值几乎相同，分别为5.4±0.6天和5.3±0.5天，且显著（p小于0.05）短于由21名健康男性组成的对照组的均值（6.9±0.3天）。得出的结论是，在血小板的体外标记中，RCD和血浆似乎同样适合作为孵育介质。
