Zhang Xiandi, Jiang Xiang'er, Wang Yun, Chen Qinzhen, Jiang Hao, Zhang Hu, Beltran Antoni, Yang Weiya, Chen Tai, Liang Chenglong, Cheng Ning, Huang Yun, Ding Guqiao, Xie Chengwang, Gao Nanfeng, Liu Juntao, Xu Wei, Huang Jinlei, Cai Dong, Zhu Lihao, Mo Songjin, Shen Mengzhe, Zhang Wenwei, Lehner Ben, Ni Ming, Wang Jian, Xu Xun, Shen Yue
BGI Research, Changzhou, China.
Guangdong Provincial Key Laboratory of Genome Read and Write, BGI Research, Shenzhen, China.
Nat Biotechnol. 2025 Oct 1. doi: 10.1038/s41587-025-02844-0.
Current high-throughput DNA synthesis technologies use intricate chip and microfluidic systems to produce large-scale synthetic oligonucleotides but with low concentration and limited compatibility for long DNA assembly. Here we report a massive-in-parallel synthesis system, with an 'identification-sorting-synthesis-recycling' iteration mechanism applied to microchips for high-throughput DNA synthesis. This approach increases DNA product concentration by four to six orders of magnitude and simplifies downstream processes for large-scale gene synthesis.
