Stephenson J R, Axelrad A A, McLeod D L, Shreeve M M
Proc Natl Acad Sci U S A. 1971 Jul;68(7):1542-6. doi: 10.1073/pnas.68.7.1542.
A culture method has been developed in which erythroid colonies are produced in vitro from hemopoietic cells from the livers of 13-day fetuses of C3H(f)/Bi mice. Heme synthesis by the cultures was correlated with the presence of these colonies, and the hemoglobin produced was shown to be electrophoretically normal. The individual colonies were identified as erythroid since they were erythropoietin-dependent, positively stained by the histochemical "Lepehne" procedure for hemoglobin, and labeled by (59)Fe radioautography. Evidence is presented that the development of these colonies is under separate control from that of granulocytic colonies found in the same cultures.
已开发出一种培养方法，可从C3H(f)/Bi小鼠13天胎儿肝脏中的造血细胞在体外产生红系集落。培养物中的血红素合成与这些集落的存在相关，并且所产生的血红蛋白经电泳显示正常。单个集落被鉴定为红系，因为它们依赖促红细胞生成素，通过血红蛋白的组织化学“勒佩内”程序呈阳性染色，并通过(59)Fe放射自显影进行标记。有证据表明，这些集落的发育与在相同培养物中发现的粒细胞集落的发育受不同的控制。
