Chui D H, Djaldetti M, Marks P A, Rifkind R A
J Cell Biol. 1971 Dec;51(3):585-95. doi: 10.1083/jcb.51.3.585.
The effect of the hormone, erythropoietin, on cultures of erythroblasts derived from the livers of fetal C57BL/6J mice was examined. An increase both in the content and in the rate of synthesis of normal adult mouse globin chains was detected in hormone-treated cultures. The rate of protein synthesis by individual erythroblasts does not increase in response to the hormone, whereas the absolute number of hemoglobin-synthesizing cells does increase and accounts for the observed stimulation of hemoglobin synthesis. The principal effect of erythropoietin appears to be upon the population of immature erythroid precursor cells which persists in the presence of the hormone, the cells maintaining their ability to replicate, and their capacity to differentiate into hemoglobinizing erythroblasts. In the absence of hormone, already committed erythroblasts continue their development, but erythropoiesis is not sustained.
研究了激素促红细胞生成素对源自胎儿C57BL/6J小鼠肝脏的成红细胞培养物的影响。在激素处理的培养物中，检测到正常成年小鼠珠蛋白链的含量和合成速率均增加。单个成红细胞的蛋白质合成速率不会因激素而增加，而合成血红蛋白的细胞的绝对数量确实增加了，这解释了观察到的血红蛋白合成的刺激现象。促红细胞生成素的主要作用似乎是作用于未成熟的红系前体细胞群体，这些细胞在激素存在的情况下持续存在，保持其复制能力以及分化为合成血红蛋白的成红细胞的能力。在没有激素的情况下，已经定向的成红细胞会继续发育，但红细胞生成无法持续。
