Markovits P, Levy S, Papadopoulo D, Nocentini S, Tripier M F, Benda P
C R Acad Hebd Seances Acad Sci D. 1977 Sep 19;285(5):623-6.
Using primary cultures originating from 14 day old fetal Hamster brain, we have obtained a cell line with glial morphology. These cell remain non transplantable during the first year of in vitro culture, but undergo spontaneous transformation during the second year. Following prolonged contact of the glial-like cells with 25 to 50 microgram/ml of methylnitrosourea (MNU), a malignant transformation is observed 5 months after the treatment. The lowest concentrations of (MNU) do not cause malignant transformation, but seem to inhibit (or postpone) the spontaneous transformation. After MNU treatment, cells retain their glial nature.
利用源自14日龄仓鼠胚胎脑的原代培养物，我们获得了一种具有神经胶质形态的细胞系。这些细胞在体外培养的第一年仍不可移植，但在第二年发生自发转化。在将类神经胶质细胞与25至50微克/毫升的甲基亚硝基脲（MNU）长时间接触后，在处理5个月后观察到恶性转化。最低浓度的（MNU）不会引起恶性转化，但似乎会抑制（或推迟）自发转化。MNU处理后，细胞保持其神经胶质特性。
