HeLa细胞核中RNA聚合酶的产物。
Products of RNA polymerases in HeLa cell nuclei.
作者信息
Zylber E A, Penman S
出版信息
Proc Natl Acad Sci U S A. 1971 Nov;68(11):2861-5. doi: 10.1073/pnas.68.11.2861.
Abstract
RNA polymerase activities in intact HeLa cell nuclei have been examined and compared to activities investigated in previous studies of the purified enzymes. The RNA synthesized by the mammalian polymerases while still in nuclei is identified. The polymerases are tentatively identified by location, sensitivity to alpha-amanitin, and response to manganese and altered ionic strength. Polymerase I is located in the nucleolus and labels partially complete precursor molecules of ribosomal RNA. Polymerases II and III are in the nucleoplasm and both label giant nuclear heterogeneous RNA.
摘要
已对完整的海拉细胞核中的RNA聚合酶活性进行了检测，并与之前对纯化酶的研究所检测的活性进行了比较。确定了哺乳动物聚合酶在细胞核内时所合成的RNA。通过位置、对α-鹅膏蕈碱的敏感性以及对锰和离子强度变化的反应，初步确定了这些聚合酶。聚合酶I位于核仁中，标记核糖体RNA的部分完整前体分子。聚合酶II和III位于核质中，二者均标记巨大的核不均一RNA。
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本文引用的文献
1
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Proc Natl Acad Sci U S A. 1962 Dec;48(12):2179-86. doi: 10.1073/pnas.48.12.2179.
2
EVIDENCE FOR TWO DNA-DEPENDENT RNA POLYMERASE ACTIVITIES IN ISOLATED RAT-LIVER NUCLEI.大鼠肝脏细胞核中两种依赖DNA的RNA聚合酶活性的证据。
Biochim Biophys Acta. 1964 Jul 22;87:531-3. doi: 10.1016/0926-6550(64)90133-1.
3
Ribonucleic acid synthesis in nuclear extracts of mammalian cells grown in suspension culture; effect of ionic strength and surface-active agents.悬浮培养的哺乳动物细胞核提取物中的核糖核酸合成；离子强度和表面活性剂的影响。
Biochim Biophys Acta. 1961 Jul 22;51:201-4. doi: 10.1016/0006-3002(61)91042-3.
4
Amino acid metabolism in mammalian cell cultures.哺乳动物细胞培养中的氨基酸代谢
Science. 1959 Aug 21;130(3373):432-7. doi: 10.1126/science.130.3373.432.
5
Use of low concentrations of actinomycin D in the study of RNA synthesis in Ehrlich ascites cells.低浓度放线菌素D在艾氏腹水癌细胞RNA合成研究中的应用。
J Mol Biol. 1966 Sep;20(1):63-73. doi: 10.1016/0022-2836(66)90117-3.
6
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7
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9
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J Mol Biol. 1966 Aug;19(2):349-61. doi: 10.1016/s0022-2836(66)80009-8.
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