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对黑腹果蝇雌性进行离心处理导致的异常卵子发生和胚胎发生。

Abnormal oogenesis and embryogenesis resulting from centrifuging Drosophila melanogaster females.

作者信息

Bownes M

出版信息

J Embryol Exp Morphol. 1977 Aug;40:65-81.

PMID:410902
Abstract

Females of Drosophila melanogaster were centrifuged at 2400 g and 4200 g for 3 h in a number of different orientations. The oocytes in various stages of vitellogenesis become separated into three layers; a centrifugal yolk layer, a central cytoplasmic layer, and a centripetal lipid layer. The direction of layering is related to the orientation of the female. The process of recovery of the ovaries was followed and the development of the eggs laid analysed. Many of the eggs laid over the subsequent 3 days die very early in development and fail to produce any differentiated structures. Some hatch into normal larvae and others produce defective embryos. The most common defect being a misarrangement of the segmentation. The differences in the kinds of experimentally inducible pattern aberrations in Drosophila and other diptera is discussed.

摘要

将黑腹果蝇的雌性个体在2400克和4200克的离心力下,以多种不同方向离心3小时。处于不同卵黄发生阶段的卵母细胞被分离成三层:离心的卵黄层、中央的细胞质层和向心的脂质层。分层方向与雌性个体的方向有关。跟踪卵巢的恢复过程并分析所产卵的发育情况。在随后3天内产下的许多卵在发育早期就死亡,无法产生任何分化结构。一些孵化成正常幼虫,另一些则产生有缺陷的胚胎。最常见的缺陷是体节排列紊乱。讨论了果蝇和其他双翅目昆虫中实验诱导的模式畸变类型的差异。

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