Eastwood G L
Surgery. 1977 Nov;82(5):613-20.
Intravenous (IV) alimentation of experimental animals has been associated with an attenuation of the small bowel mucosa. To determine whether alterations in epithelial renewal accompany the morphological changes, we studied cell proliferation as well as the light and electron microscopic appearance of the small bowel in intravenously and orally alimented rabbits. After 10 days of either IV or oral alimentation, 3H-thymidine was given intravenously to all animals to label proliferating cells, and 1 hour later sections of duodenum, upper jejunum, and distal ileum were taken for autoradiography and light and electron microscopy. IV alimentation was accompanied by significant reductions in epithelial cell proliferation and mucosal thickness in the proximal small bowel. The density of goblet cells was greater in the duodenum and jejunum, but less in the ileum, of intravenously alimented rabbits than in corresponding areas of the oral controls. Furthermore, ultrastructural changes in the villous absorptive cells of intravenously alimented rabbits, such as lengthened microvilli and increased numbers of multivesicular bodies, suggested that epithelial migration also may be slowed during IV alimentation.
对实验动物进行静脉营养支持与小肠黏膜萎缩有关。为了确定上皮更新的改变是否伴随形态学变化，我们研究了静脉营养和经口营养的家兔小肠的细胞增殖以及光镜和电镜表现。在进行10天的静脉营养或经口营养后，给所有动物静脉注射³H-胸腺嘧啶核苷以标记增殖细胞，1小时后取十二指肠、空肠上段和回肠末端切片进行放射自显影以及光镜和电镜检查。静脉营养伴随着近端小肠上皮细胞增殖和黏膜厚度的显著降低。与经口营养的对照家兔相应部位相比，静脉营养家兔十二指肠和空肠的杯状细胞密度更高，但回肠的杯状细胞密度更低。此外，静脉营养家兔绒毛吸收细胞的超微结构变化，如微绒毛延长和多泡体数量增加，提示静脉营养期间上皮迁移也可能减慢。
