Williams J E, Whittemore A D
Appl Microbiol. 1972 May;23(5):931-7. doi: 10.1128/am.23.5.931-937.1972.
A sensitive antiglobulin (AG) test procedure for the demonstration and experimental study of the agglutinin response of chickens infected orally with Salmonella typhimurium is described. A tetrazolium-stained S. typhimurium antigen was employed with microagglutination techniques and equipment for the first time in conducting the AG test. Results with the conventional macroscopy tube agglutination test for S. typhimurium and the 24-hr microtest were comparable; however, the AG test enhanced titers as much as 16 times, and these persisted at a significant level for as long as 4 months. This study is being extended to other Salmonella serotypes and possible field applications of the AG test procedure.
本文描述了一种灵敏的抗球蛋白（AG）检测方法，用于对口服感染鼠伤寒沙门氏菌的鸡的凝集素反应进行演示和实验研究。在进行AG检测时，首次将四唑盐染色的鼠伤寒沙门氏菌抗原与微凝集技术和设备一起使用。鼠伤寒沙门氏菌的传统宏观试管凝集试验结果与24小时微量试验结果相当；然而，AG检测使效价提高了多达16倍，并且这些效价在长达4个月的时间内一直保持在显著水平。这项研究正在扩展到其他沙门氏菌血清型以及AG检测程序可能的现场应用。
