Metzgar R S, Mohanakumar T
Am J Clin Pathol. 1977 Nov;68(5 Suppl):699-705.
Nonhuman primate antisera to human leukemia-associated antigens and to normal T- and B-lymphocyte antigens are useful reagents for detecting and classifying leukemic cells in a microcytotoxicity assay. These antisera are able to distinguish lymphocytic from myeloid leukemia cells, and can also identify acute myelomonocytic leukemia and chronic myelocytic leukemia blastic crisis cells from cells of other types of leukemia. The advantages and disadvantages of the reagents in the nosology and clinical management of leukemia patients are discussed in depth. However, despite certain shortcomings in the microcytotoxicity assays, the reagents and method do provide the necessary tools for antigenic isolation and identification. Purified antigen may then serve to open new horizons in the detection, diagnosis, classification and perhaps therapy of human leukemia.
针对人类白血病相关抗原以及正常T和B淋巴细胞抗原的非人灵长类动物抗血清，是在微量细胞毒性试验中检测和分类白血病细胞的有用试剂。这些抗血清能够区分淋巴细胞性白血病细胞和髓细胞性白血病细胞，还能从其他类型白血病细胞中识别急性粒单核细胞白血病细胞和慢性粒细胞白血病急变期细胞。深入讨论了这些试剂在白血病患者疾病分类学和临床管理中的优缺点。然而，尽管微量细胞毒性试验存在某些缺点，但这些试剂和方法确实为抗原分离和鉴定提供了必要工具。纯化的抗原可能会为人类白血病的检测、诊断、分类乃至治疗开辟新的前景。
