Farquhar M G, Bainton D F, Baggiolini M, de Duve C
J Cell Biol. 1972 Jul;54(1):141-56. doi: 10.1083/jcb.54.1.141.
When rabbit peritoneal exudates (97% polymorphonuclear [PMN] leukocytes, 2% mononuclear cells) were fractionated by zonal sedimentation or isopycnic centrifugation, four fractions (A, B, C, and D) were obtained, as reported earlier. "A" consisted largely of PMN azurophil granules, "B" of PMN specific granules, and "D" of membranous elements. The source of the more heterogeneous "C" fraction (containing acid hydrolases) was uncertain. To gain further information on the nature of this fraction, cytochemical tests for acid phosphatase (AcPase) were carried out on the starting cells and on the fractions. In intact PMN, lead phosphate reaction product was found in Golgi complexes, perinuclear cisternae, and some azurophil granules (immature forms or disrupted mature forms) of a few cells. The specifics and the intact azurophils were not reactive. Reaction product was also found within Golgi cisternae, secondary lysosomes, and some of the azurophil granules of mononuclear cells. Observations on the A and B fractions confirmed those in situ regarding the localization of reaction product in disrupted PMN azurophils, its absence from specifics, and the latency of the enzyme activity in intact azurophils. In the C fraction, AcPase was found in three structures (a) Golgi cisternae, (b) dense bodies, and (c) small pleomorphic granules Comparison with the starting cells indicates that the Golgi complexes are probably derived from both PMN leukocytes and mononuclear cells, whereas the remaining elements resemble (in size, shape, and density) secondary lysosomes and azurophil granules of mononuclear cells. The results indicate that the bulk of the cytochemically detectable AcPase present in the C fraction is derived from mononuclear cells, rather than from PMN leukocytes
如先前报道,当兔腹膜渗出液(97%为多形核[PMN]白细胞,2%为单核细胞)通过区带沉降或等密度离心进行分级分离时,获得了四个组分(A、B、C和D)。“A”主要由PMN嗜天青颗粒组成,“B”由PMN特异性颗粒组成,“D”由膜性成分组成。更为不均一的“C”组分(含有酸性水解酶)的来源尚不确定。为了进一步了解该组分的性质,对起始细胞和各组分进行了酸性磷酸酶(AcPase)的细胞化学检测。在完整的PMN中,磷酸铅反应产物见于高尔基体复合体、核周池以及少数细胞的一些嗜天青颗粒(未成熟形式或破裂的成熟形式)中。特异性颗粒和完整的嗜天青颗粒无反应性。在单核细胞的高尔基池、次级溶酶体以及一些嗜天青颗粒中也发现了反应产物。对A和B组分的观察证实了原位观察结果,即反应产物在破裂的PMN嗜天青颗粒中的定位、在特异性颗粒中的缺失以及完整嗜天青颗粒中酶活性的潜伏性。在C组分中,AcPase存在于三种结构中:(a)高尔基池,(b)致密体,(c)小的多形性颗粒。与起始细胞相比表明,高尔基复合体可能来源于PMN白细胞和单核细胞两者,而其余成分(在大小、形状和密度上)类似于单核细胞的次级溶酶体和嗜天青颗粒。结果表明,C组分中细胞化学可检测到的大部分AcPase来源于单核细胞,而非PMN白细胞。