Maitra S K, Yoshikawa T T, Hansen J L, Nilsson-Ehle I, Palin W J, Schotz M C, Guze L B
Clin Chem. 1977 Dec;23(12):2275-8.
We describe a high-performance liquid chromatographic method for the quantitative determination of gentamicin in serum. The antibiotic was separated from serum by passage through a silicic acid column, derivatized with o-phthalaldehyde, and eluted with ethanol. The derivatized gentamicin was then separated into all three of its major components by reversed-phase chromatography and quantified by fluorometry. Concentrations in serum as low as 0.5 mg of gentamicin per liter could be accurately determined. A standard curve showed a linear response for serum containing gentamicin at concentrations ranging from 0 to 20 mg/liter. Tobramycin, amikacin, ampicillin, penicillin G, methicillin, carbenicillin, chloramphenicol, clindamycin, and cephalothin did not interfere with the gentamicin assay. Comparison with an accepted microbiological assay yielded a correlation coefficient of 0.99. This chemical assay is rapid (less than 30 min), sensitive, accurate, specific, and appears to be applicable to other aminoglycosides.
我们描述了一种用于定量测定血清中庆大霉素的高效液相色谱法。抗生素通过硅胶柱从血清中分离出来，用邻苯二甲醛衍生化，并用乙醇洗脱。然后，通过反相色谱法将衍生化的庆大霉素分离成其所有三种主要成分，并通过荧光法进行定量。血清中低至每升0.5毫克的庆大霉素浓度都可以准确测定。标准曲线显示，对于浓度范围为0至20毫克/升的含庆大霉素血清，呈线性响应。妥布霉素、阿米卡星、氨苄西林、青霉素G、甲氧西林、羧苄西林、氯霉素、克林霉素和头孢噻吩不干扰庆大霉素测定。与公认的微生物测定法比较，相关系数为0.99。这种化学测定法快速（少于30分钟）、灵敏、准确、特异，并且似乎适用于其他氨基糖苷类药物。
