采用高效液相色谱法测定血清中的阿米卡星。
Amikacin assay in serum by high-performance liquid chromatography.
作者信息
Maitra S K, Yoshikawa T T, Steyn C M, Guze L B, Schotz M C
出版信息
Antimicrob Agents Chemother. 1978 Dec;14(6):880-5. doi: 10.1128/AAC.14.6.880.
A high-performance liquid chromatographic method for the quantitative determination of amikacin in serum is described. The antibiotic was separated from serum by adsorption on silica gel. The adsorbed drug was then derivatized with o-phthalaldehyde and eluted with ethanol. The derivatized amikacin was separated by reverse-phase, high-performance liquid chromatography and quantitated by fluorometry. A linear response for serum samples containing amikacin ranging from 1.0 to 15 mug/ml was obtained. Other antibiotics including various aminoglycosides did not interfere with the amikacin assay. Comparison with a standard microbiological assay gave a correlation coefficient of 0.99. This chemical assay is sensitive and specific under the conditions tested and can be performed rapidly.
本文描述了一种用于定量测定血清中阿米卡星的高效液相色谱法。通过硅胶吸附将抗生素从血清中分离出来。然后将吸附的药物用邻苯二甲醛衍生化，并用乙醇洗脱。衍生化的阿米卡星通过反相高效液相色谱法分离，并用荧光法进行定量。对于含阿米卡星浓度范围为1.0至15微克/毫升的血清样品，获得了线性响应。包括各种氨基糖苷类在内的其他抗生素不干扰阿米卡星的测定。与标准微生物测定法比较，相关系数为0.99。在测试条件下，这种化学测定法灵敏且特异，并且可以快速进行。
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