从滚瓶培养物中大量制备鼠源核糖核酸肿瘤病毒的纯化方法。
Purification of large amounts of murine ribonucleic acid tumor viruses produced in roller bottle cultures.
作者信息
Syrewicz J J, Naso R B, Wang C S, Arlinghaus R B
出版信息
Appl Microbiol. 1972 Sep;24(3):488-94. doi: 10.1128/am.24.3.488-494.1972.
Abstract
Murine ribonucleic acid tumor viruses and C-type virus particles are produced in relatively large quantities in roller bottle cultures. The viruses present in large volumes of culture fluids can be purified by a simple two-step procedure involving polyethylene glycol precipitation and equilibrium centrifugation in sucrose density gradients.
摘要
小鼠核糖核酸肿瘤病毒和C型病毒颗粒在滚瓶培养中大量产生。存在于大量培养液中的病毒可通过一个简单的两步程序进行纯化，该程序包括聚乙二醇沉淀和在蔗糖密度梯度中进行平衡离心。
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