Maxwell S, Arlinghaus R B
J Virol. 1981 Sep;39(3):963-7. doi: 10.1128/JVI.39.3.963-967.1981.
Moloney murine leukemia virus, disrupted in concentrations of 0.1 to 0.5% Nonidet P-40, catalyzed the cleavage of p65, the gag gene polyprotein of the Gazdar strain of murine sarcoma virus, into polypeptides with sizes and antigenic determinants of murine leukemia virus-specified p30, p15, pp12, and p10. Cleavage performed in the presence of 0.15% Nonidet P-40 in water yielded polypeptides of approximately 40,000 (P40) and 25,000 (P25) Mr. In vitro cleavage performed in a buffered solution containing dithiothreitol in addition to 0.1% Nonidet P-40 allowed the efficient processing of P40 to p30 and a band migrating with p10. Immunoprecipitation with monospecific sera indicated that P40 contained p30 and p10, whereas P25 contained p15 and pp12 determinants. P40 and P25 are similar in size and antigenic properties to Pr40gag and Pr25gag observed in infected cells (Naso et al, J. Virol. 32:187-198, 1979).
莫洛尼鼠白血病病毒在浓度为0.1%至0.5%的非离子去污剂P - 40中被破坏后，催化了鼠肉瘤病毒加兹达尔株的gag基因多聚蛋白p65裂解为具有鼠白血病病毒指定的p30、p15、pp12和p10大小及抗原决定簇的多肽。在水中0.15%非离子去污剂P - 40存在下进行的裂解产生了分子量约为40,000（P40）和25,000（P25）的多肽。除了0.1%非离子去污剂P - 40外，在含有二硫苏糖醇的缓冲溶液中进行的体外裂解使得P40高效加工为p30以及一条与p10一起迁移的条带。用单特异性血清进行免疫沉淀表明，P40含有p30和p10，而P25含有p15和pp12决定簇。P40和P25在大小和抗原特性上与在感染细胞中观察到的Pr40gag和Pr25gag相似（纳索等人，《病毒学杂志》32:187 - 198，1979年）。
