传染性胰腺坏死病毒的电子显微镜及生化特性研究
Electron microscopical and biochemical characterization of infectious pancreatic necrosis virus.
作者信息
Kelly R K, Loh P C
出版信息
J Virol. 1972 Oct;10(4):824-34. doi: 10.1128/JVI.10.4.824-834.1972.
An electron microscopical and biochemical examination of the properties of infectious pancreatic necrosis virus (IPN) and of its ribonucleic acid (RNA) was made. The buoyant density of IPN in CsCl was found to be 1.33 g/cm(3). Electron microscopical examination of the banded virus revealed structures similar in size (74 nm) and shape to reoviruses but lacking a characteristic inner capsid structure. Polyacrylamide gel electrophoretic analysis of IPN-RNA revealed a single non-segmented component of molecular weight 3.2 x 10(6). Its susceptibility to ribonuclease, base composition, and resistance to thermal denaturation indicated a single-stranded RNA structure. However, its sedimentation behavior (16S) independent of ionic strength in sucrose gradients, partial solubility in 2 m LiCl, and ribonuclease resistance in the presence of Mg(2+) suggest an unusual secondary structure of unknown nature. The accumulated data indicate that IPN virus does not belong to either the picornavirus or reovirus groups and may represent a new group of viruses.
对传染性胰腺坏死病毒（IPN）及其核糖核酸（RNA）的特性进行了电子显微镜和生化检查。发现IPN在CsCl中的浮力密度为1.33 g/cm³。对经密度梯度离心分离的病毒进行电子显微镜检查，发现其结构在大小（74纳米）和形状上与呼肠孤病毒相似，但缺乏特征性的内衣壳结构。对IPN-RNA进行聚丙烯酰胺凝胶电泳分析，显示出一个分子量为3.2×10⁶的单一非分段成分。它对核糖核酸酶的敏感性、碱基组成以及对热变性的抗性表明其为单链RNA结构。然而，其在蔗糖梯度中与离子强度无关的沉降行为（16S）、在2 m LiCl中的部分溶解性以及在Mg²⁺存在下对核糖核酸酶的抗性表明其具有性质未知的异常二级结构。积累的数据表明，IPN病毒既不属于微小核糖核酸病毒组也不属于呼肠孤病毒组，可能代表一类新的病毒。
Zotero 插件