Macdonald R D, Yamamoto T
J Gen Virol. 1977 Feb;34(2):235-47. doi: 10.1099/0022-1317-34-2-235.
The RNA from infectious pancreatic necrosis virus has been purified and had a sedimentation velocity of 14S on sucrose gradients, a buoyant density of 1-60 g/ml in CS2SO4 and pyrimidine to purine ratios near unity. The RNA had the appearance of a linear double stranded molecule with an average length of 0-92 mum and a standard deviation of 0-07 mum when observed under the electron microscope using the Kleinschmidt protein film technique. This would correspond to a mol. wt. of 2-4 +/- 0-2 X 10(6). The RNase A resistance of IPN virus RNA exhibited a marked salt dependence; it was 92% resistant in 0-1 M-NaCl, but only 9% resistant, or less, in 0-*1 M-NaCl. The RNA was resistant to denaturation by boilding at NaCl concentrations of 0-04 M or higher, but did denature at lower concentrations. Polyacrylamide gel electrophoresis of the RNA indicated that two RNA species were present and the standard deviation of lengths in the electron microscope indicated that they could not differ by more than 4 X 10(5) in mol. wt.
传染性胰腺坏死病毒的RNA已被纯化，在蔗糖梯度中的沉降速度为14S，在Cs2SO4中的浮力密度为1.60g/ml，嘧啶与嘌呤的比例接近1。当使用克莱因施密特蛋白膜技术在电子显微镜下观察时，该RNA呈现出线性双链分子的外观，平均长度为0.92μm，标准差为0.07μm。这相当于分子量为2.4±0.2×10⁶。IPN病毒RNA对核糖核酸酶A的抗性表现出明显的盐依赖性；在0.1M NaCl中92%具有抗性，但在0.01M NaCl中仅9%或更低具有抗性。在NaCl浓度为0.04M或更高时，RNA对煮沸变性具有抗性，但在较低浓度下会变性。RNA的聚丙烯酰胺凝胶电泳表明存在两种RNA，电子显微镜下长度的标准差表明它们的分子量差异不超过4×10⁵。
