Furness J B, Costa M, Blessing W W
Histochem J. 1977 Nov;9(6):745-50. doi: 10.1007/BF01003068.
Perfusion with a mixture of formaldehyde (4%) and glutaraldehyde (0.5%) is shown both to fix central nervous tissue and to produce, with no further treatment, a fluorescence histochemical localization of catecholamines. After perfusion, serial sections can be readily taken through the whole brain with a Vibratome. Landmarks which are apparent at low power with white-light illumination can be seen when the sections are viewed in the fluorescence microscope. Catecholamine-containing nerve cell bodies, varicose axon terminals and non-varicose nerve fibres appear brightly fluorescent and well localized. The method has been applied to rats, guinea-pigs and rabbits and is ideally suited to the accurate mapping of central catecholamine neurons and their processes.
用4%甲醛和0.5%戊二醛的混合液灌注，已证明既能固定中枢神经组织，又能在无需进一步处理的情况下产生儿茶酚胺的荧光组织化学定位。灌注后，可用振动切片机轻松地切取全脑的连续切片。在荧光显微镜下观察切片时，可以看到在白光照明下低倍镜下明显的地标。含儿茶酚胺的神经细胞体、曲张的轴突终末和无曲张的神经纤维呈现明亮的荧光且定位良好。该方法已应用于大鼠、豚鼠和兔子，非常适合对中枢儿茶酚胺神经元及其突起进行精确绘图。
