Furness J B, Heath J W, Costa M
Histochemistry. 1978 Sep 28;57(4):285-95. doi: 10.1007/BF00492664.
Aqueous solutions combining a high concentration of formaldehyde (4%) with low concentrations of glutaraldehyde (0.5--01%) have been used to simultaneously localize amines by the formation of fluorescent products and to fix central nervous tissue for electron microscopy. The fluorescence reaction is produced by the aldehyde mixture at room temperature and the fluorescence is stable when the tissue is maintained in aqueous solution. This means that nerve cell bodies and terminal fields which contain catecholamines can be located accurately in vibratome sections at the light microscope level and, after further processing, can be examined under the electron microscope. With 1% glutaraldehyde in the aldehyde mixture, ultrastructural details are well preserved; there is no significant distortion of any component of the tissue. If vibratome or cryostat sections are dried against glass slides, the intensity of the fluorescence reaction is enhanced and the sections can be permanently mounted.
将高浓度甲醛（4%）与低浓度戊二醛（0.5 - 0.1%）混合的水溶液已被用于通过形成荧光产物同时定位胺类，并固定中枢神经组织用于电子显微镜检查。醛混合物在室温下产生荧光反应，当组织保持在水溶液中时荧光稳定。这意味着含有儿茶酚胺的神经细胞体和终末区域可以在光镜水平的振动切片机切片中准确定位，经过进一步处理后，可以在电子显微镜下检查。醛混合物中含有1%戊二醛时，超微结构细节能得到很好的保存；组织的任何成分都没有明显变形。如果将振动切片机或低温恒温器切片贴在载玻片上干燥，荧光反应强度会增强，切片可以永久封固。
