酪氨酸羟化酶与其他细胞质成分相关的快速轴浆运输。
Rapid axoplasmic transport of tyrosine hydroxylase in relation to other cytoplasmic constituents.
作者信息
Jarrott B, Geffen L B
出版信息
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3440-2. doi: 10.1073/pnas.69.11.3440.
The transport of norepinephrine and two key enzymes involved in its synthesis, tyrosine hydroxylase (EC 1.14.3a) and dopamine beta-hydroxylase (EC 1.14.2.1), has been studied in relation to other axonal constituents in ligated chicken sciatic nerves. Norepinephrine, tyrosine hydroxylase, and dopamine beta-hydroxylase activity all increased proximal to the constriction over a 20-hr period. The rate of transport of norepinephrine, tyrosine hydroxylase, and dopamine beta-hydroxylase were calculated as 2.6, 1.9, and 3.4 mm/hr, respectively. The only enzyme marker to show a similar rate of accumulation was acetylcholinesterase (EC 3.1.1.7), a putative marker for endoplasmic reticulum. The rapid axoplasmic transport of tyrosine hydroxylase from the cell bodies to the terminals of sympathetic neurons may be adequate to account for the elevated amounts of this enzyme in chronically active axon terminals, without the necessity to invoke peripheral axonal synthesis of the enzyme to explain such elevated amounts.
在结扎的鸡坐骨神经中，已对去甲肾上腺素及其合成过程中涉及的两种关键酶——酪氨酸羟化酶（EC 1.14.3a）和多巴胺β-羟化酶（EC 1.14.2.1）与其他轴突成分的运输进行了研究。在20小时的时间段内，去甲肾上腺素、酪氨酸羟化酶和多巴胺β-羟化酶的活性在缩窄部位近端均有所增加。去甲肾上腺素、酪氨酸羟化酶和多巴胺β-羟化酶的运输速率分别计算为2.6、1.9和3.4毫米/小时。唯一显示出相似积累速率的酶标志物是乙酰胆碱酯酶（EC 3.1.1.7），它是内质网的一种假定标志物。酪氨酸羟化酶从交感神经元的细胞体到终末的快速轴浆运输，可能足以解释在长期活跃的轴突终末中该酶含量的升高，而无需借助该酶的外周轴突合成来解释这种升高的含量。
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