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牛蛙坐骨神经酪氨酸羟化酶和多巴胺-β-羟化酶的免疫荧光及生化研究

Immunofluorescent and biochemical studies on tyrosine hydroxylase and dopamine-beta-hydroxylase of the bullfrog sciatic nerves.

作者信息

Nagatsu I, Kondo Y, Inagaki S, Kojima H, Nagatsu T

出版信息

Histochemistry. 1979 Jun 18;61(2):103-9. doi: 10.1007/BF00496522.

DOI:10.1007/BF00496522
PMID:88431
Abstract

Immunofluorescence specific for tyrosine hydroxylase (TH) or dopamine-beta-hydroxylase (DBH) was accentuated in both proximal and distal segments of the sciatic nerve after ligation. Estimations of the enzyme activities confirmed the above results. Mean axoplasmic flow rates of TH and DBH in bullfrog sciatic nerve were found to be 8 and 123 mm/day, respectively. They were decreased by colchicine or by cold temperatures (4 degrees C).

摘要

结扎后,坐骨神经近端和远端段中酪氨酸羟化酶(TH)或多巴胺-β-羟化酶(DBH)的免疫荧光均增强。酶活性测定证实了上述结果。牛蛙坐骨神经中TH和DBH的平均轴浆流速分别为8毫米/天和123毫米/天。秋水仙碱或低温(4摄氏度)会使其降低。

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Immunofluorescent and biochemical studies on tyrosine hydroxylase and dopamine-beta-hydroxylase of the bullfrog sciatic nerves.牛蛙坐骨神经酪氨酸羟化酶和多巴胺-β-羟化酶的免疫荧光及生化研究
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本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
The anatomical characteristics of dopamine-beta-hydroxylase accumulation in ligated sciatic nerve.
J Histochem Cytochem. 1974 Nov;22(11):1010-8. doi: 10.1177/22.11.1010.
3
A simple and sensitive assay for dopamine-beta-hydroxylase activity by dual-wavelength spectrophotometry.一种通过双波长分光光度法检测多巴胺-β-羟化酶活性的简单灵敏的测定方法。
Biochem Med. 1974 Aug;10(4):320-8. doi: 10.1016/0006-2944(74)90035-0.
4
Axonal transport of phenylethanolamine-N-methyltransferase in toad sciatic nerve.
J Neurochem. 1974 Jun;22(6):1059-64. doi: 10.1111/j.1471-4159.1974.tb04337.x.
5
The effect of colchicine on the transport of axonal protein in the chicken.秋水仙碱对鸡轴突蛋白运输的影响。
Biochem J. 1970 May;117(4):767-71. doi: 10.1042/bj1170767.
6
Relationship between the rate of axoplasmic transport and subcellular distribution of enzymes involved in the synthesis of norepinephrine.去甲肾上腺素合成过程中相关酶的轴浆运输速率与亚细胞分布之间的关系。
J Neurochem. 1973 Jun;20(6):1691-706. doi: 10.1111/j.1471-4159.1973.tb00285.x.
7
Axonal transport of catecholamine synthesizing and metabolizing enzymes.儿茶酚胺合成与代谢酶的轴突运输
J Neurochem. 1973 May;20(5):1361-71. doi: 10.1111/j.1471-4159.1973.tb00248.x.
8
Transport and turnover of dopamine- -hydroxylase (EC 1.14.2.1) in sympathetic nerves of the rat.
J Neurochem. 1972 Sep;19(9):2183-93. doi: 10.1111/j.1471-4159.1972.tb05127.x.
9
Determination of protein: a modification of the Lowry method that gives a linear photometric response.蛋白质的测定:一种改进的洛瑞法,可产生线性光度响应。
Anal Biochem. 1972 Aug;48(2):422-7. doi: 10.1016/0003-2697(72)90094-2.
10
Early changes in the structure of rat sciatic nerves by ligation.结扎对大鼠坐骨神经结构的早期影响。
Arch Histol Jpn. 1978 Nov;41(5):439-51. doi: 10.1679/aohc1950.41.439.