Immunofluorescent and biochemical studies on tyrosine hydroxylase and dopamine-beta-hydroxylase of the bullfrog sciatic nerves.

Immunofluorescence specific for tyrosine hydroxylase (TH) or dopamine-beta-hydroxylase (DBH) was accentuated in both proximal and distal segments of the sciatic nerve after ligation. Estimations of the enzyme activities confirmed the above results. Mean axoplasmic flow rates of TH and DBH in bullfrog sciatic nerve were found to be 8 and 123 mm/day, respectively. They were decreased by colchicine or by cold temperatures (4 degrees C).