分解代谢物阻遏对乳糖操纵子的多顺反子效应。
Polycistronic effects of catabolite repression on the lac operon.
作者信息
Silverstone A E, Magasanik B
出版信息
J Bacteriol. 1972 Dec;112(3):1184-92. doi: 10.1128/jb.112.3.1184-1192.1972.
Abstract
The catabolite repression caused by glucose and glucose-6-phosphate has been studied for both beta-galactosidase and thiogalactoside transacetylase, the products of the operator proximal and distal cistrons of the lac operon, respectively. We find that both cistrons are affected coordinately by this form of repression. We also find that a single alteration at the lac promoter region is sufficient to abolish sensitivity to repression of both cistrons. From this, we conclude that there is only one target site for catabolite repression in the lac operon.
摘要
葡萄糖和6-磷酸葡萄糖引起的分解代谢物阻遏作用，已针对β-半乳糖苷酶和硫代半乳糖苷转乙酰基酶进行了研究，这两种酶分别是乳糖操纵子中操纵基因近端和顺反子远端的产物。我们发现这两个顺反子都受到这种阻遏形式的协同影响。我们还发现，乳糖启动子区域的单一改变足以消除对这两个顺反子阻遏的敏感性。由此，我们得出结论，在乳糖操纵子中，分解代谢物阻遏只有一个靶位点。
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本文引用的文献
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