Schneider C H, de Weck A L, Schenkel E, Wirz W, Lazary S
Immunology. 1972 Dec;23(6):911-20.
The immunogenicity of a series of dodecapeptides was evaluated in random-bred guinea-pigs. The peptides were synthesized by an economical two-phase approach specifically developed for the production of gram-amounts of peptide antigens. The peptides contain L-leucine, L-alanine, glycine and L-lysine and differ from each other by number and distribution of lysine residues. Some of the peptides carry DNP groups at their N-terminus. With the exception of one (5,11-Lys-LAG-OEt), all peptides induced a state of cellular immunity as evaluated by delayed dermal reactions and macrophage migration inhibition. Some peptides also induced the formation of circulating antibodies. Immunogenicity appears to increase with the number of basic groups and is more pronounced with DNP peptides. Delayed dermal responses are highly specific since even peptides closely related to the immunogen did not elicit reactions. Reactions were however observed when some mixtures of closely related peptides not used for immunizing the animal were administered. This `antigenic cooperation' effect is discussed briefly.
在随机繁殖的豚鼠中评估了一系列十二肽的免疫原性。这些肽是通过专门开发的一种经济的两相方法合成的，该方法用于生产克级量的肽抗原。这些肽含有L-亮氨酸、L-丙氨酸、甘氨酸和L-赖氨酸，彼此之间赖氨酸残基的数量和分布不同。一些肽在其N端带有DNP基团。除一种肽（5,11-Lys-LAG-OEt）外，通过迟发性皮肤反应和巨噬细胞迁移抑制评估，所有肽均诱导了细胞免疫状态。一些肽还诱导了循环抗体的形成。免疫原性似乎随着碱性基团数量的增加而增强，并且对于DNP肽更为明显。迟发性皮肤反应具有高度特异性，因为即使与免疫原密切相关的肽也不会引发反应。然而，当给予一些未用于免疫动物的密切相关肽的混合物时，观察到了反应。简要讨论了这种“抗原协同”效应。
