Zohner A, Broda E
Orig Life. 1979 Sep;9(4):291-8. doi: 10.1007/BF00926822.
In experiments on the prebiotic formation of nitric oxides, anoxic mixtures of N2 and water vapour were sparked in contact with phosphate buffer solutions at various pH values. Nitrite was found in the aqueous phase, and nitrate grew from it, presumably by reaction with H2O2. In acid solutions, these anions were reduced and destroyed by Fe2+, and the same was true of nitrite in solutions kept at a pH value similar to that of the contemporary ocean (8.2) with HEPES buffer. Nitrate was not destroyed in short-term experiments, but as in sparking nitrate is formed only vianitrite, neither anion could accumulate. In further sparking experiments with alkaline sulphide, both nitrite and nitrate were reduced entirely. It is concluded that it is unlikely that the primeval ocean contained appreciable concentrations of nitrite or nitrate either at the reducing or at the redox-neutral stage.
在关于一氧化氮益生元形成的实验中，将氮气与水蒸气的缺氧混合物与不同pH值的磷酸盐缓冲溶液接触进行火花放电。在水相中发现了亚硝酸盐，并且硝酸盐可能是通过与过氧化氢反应从亚硝酸盐中生成的。在酸性溶液中，这些阴离子会被亚铁离子还原并破坏，在使用HEPES缓冲液保持pH值与现代海洋（8.2）相似的溶液中的亚硝酸盐也是如此。在短期实验中硝酸盐没有被破坏，但由于在火花放电中硝酸盐仅通过亚硝酸盐形成，所以这两种阴离子都无法积累。在进一步使用碱性硫化物的火花放电实验中，亚硝酸盐和硝酸盐都被完全还原。结论是，在还原阶段或氧化还原中性阶段，原始海洋中不太可能含有可观浓度的亚硝酸盐或硝酸盐。
