Tessler J
Can J Comp Med. 1973 Apr;37(2):207-9.
Two species of ruminant mycoplasma colonies had to be fixed in ethyl alcohol so that incident immunofluorescence method could be applied. In addition, the stain reaction had to be kept for 90 minutes at 37 degrees C. This fluorescent antibody (FA) method was developed to identify colonies of Vom strain of Mycoplasma mycoides var. capri, V-5 strain of M. mycoides var. mycoides, and PG-2 strain of M. agalactiaeon agar, using fluorescent ultraviolet light. Fluorescence was not demonstrated when heterologous conjugates or normal rabbit serum conjugate were applied but the reaction appeared to be specific for each strain of mycoplasma. The FA method was able to differentiate specific mycloplasma colonies in mixed cultures.
两种反刍动物支原体菌落必须用乙醇固定，以便能够应用间接免疫荧光法。此外，染色反应必须在37摄氏度下保持90分钟。这种荧光抗体（FA）方法是为了利用荧光紫外线来鉴定丝状支原体山羊亚种Vom株、丝状支原体丝状亚种V-5株和无乳支原体PG-2株在琼脂上的菌落而开发的。当应用异源结合物或正常兔血清结合物时未显示荧光，但该反应似乎对每种支原体菌株具有特异性。FA方法能够在混合培养物中区分特定的支原体菌落。
