Çibuk Salih, Sarikaya Mücahit, Ulema Mustafa Sencer, Pancar Zarife, Öğe Beyza, Embiyaoğlu Nuri Mert, Aslan Mehdi, Aydemir İsa, Keskin Kadir, Yarayan Yunus Emre, Grivas Gerasimos V, Alkhamees Nouf H, Sheeha Bodor Bin, Al-Mhanna Sameer Badri, Badicu Georgian, Batrakoulis Alexios
Vocational School of Health Services, Van Yuzuncu Yıl University, Van, Turkey.
Department of Physical Education and Sports Teaching, Van Yüzüncü Yıl University, Van, Turkey.
Sci Rep. 2025 Nov 12;15(1):39583. doi: 10.1038/s41598-025-25470-x.
Chromium picolinate influences antioxidant mechanisms, potentially affecting oxidative stress levels during prolonged aerobic exercise. This study investigates the effects of chromium supplementation on Catalase (CAT) activity and Malondialdehyde (MDA), advanced oxidation protein products (AOPP), and reduced glutathione (GSH) levels in chronic aerobic exercise in male rats. Twenty-eight male Wistar albino rats were divided into 4 groups containing 7 mice each (control, exercise, chromium, and chromium + exercise). Supplemented rats received chromium picolinate (8 µg/kg/day) daily for eight weeks. Exercise was performed on a rat treadmill at an average speed of 15 cm/s for 20 min, five days a week for eight weeks. At the end of the 8th week of the experimental period, blood samples were taken. CAT, MDA, AOPP, and GSH analyses were performed. It was observed that the chromium + exercise group induced a significant reduction in CAT activity compared to the other three groups (- 8.6 to - 12%, p < 0.05). MDA values meaningfully increased (18.2-25.7%, p < 0.001) in all groups, except the controls after the 8-week intervention. All groups demonstrated an increase in AOPP (8.1-12.3%, p < 0.001), but not the controls. In GSH, all experimental groups showed a significant elevation (30.3-45.8%, p < 0.001) compared to the control group (p < 0.001) following an 8-week intervention period. The present findings indicate that supplementation with chromium picolinate, whether administered alone or in conjunction with aerobic exercise, led to modulations in oxidative stress and redox status indices in male rats following an 8-week aerobic exercise regimen. The observed reduction in CAT activity may suggest a lowered oxidative challenge; however, this finding should be interpreted with caution, as decreased antioxidant enzyme activity can also reflect a potential limitation in defense capacity.
吡啶甲酸铬会影响抗氧化机制，可能会在长时间有氧运动期间影响氧化应激水平。本研究调查了补充铬对雄性大鼠慢性有氧运动中过氧化氢酶（CAT）活性、丙二醛（MDA）、晚期氧化蛋白产物（AOPP）和还原型谷胱甘肽（GSH）水平的影响。将28只雄性Wistar白化大鼠分为4组，每组7只（对照组、运动组、铬组和铬+运动组）。补充组大鼠连续八周每天接受吡啶甲酸铬（8微克/千克/天）。在大鼠跑步机上以平均15厘米/秒的速度进行运动20分钟，每周五天，共八周。在实验期第8周结束时，采集血样。进行CAT、MDA、AOPP和GSH分析。观察到，与其他三组相比，铬+运动组的CAT活性显著降低（-8.6%至-12%，p<0.05）。在8周干预后，除对照组外，所有组的MDA值均显著增加（18.2%-25.7%，p<0.001）。所有组的AOPP均有所增加（8.1%-12.3%，p<0.001），但对照组除外。在GSH方面，经过8周的干预期后，所有实验组与对照组相比均显著升高（30.3%-45.8%，p<0.001）（p<0.001）。目前的研究结果表明，在进行8周有氧运动方案后，单独或与有氧运动联合施用吡啶甲酸铬会导致雄性大鼠氧化应激和氧化还原状态指标的调节。观察到的CAT活性降低可能表明氧化挑战降低；然而，这一发现应谨慎解读，因为抗氧化酶活性降低也可能反映防御能力的潜在限制。
