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I型专性甲基营养菌荚膜甲基球菌对(14C)乙酸盐的同化作用

(14C)acetate assimilation by a type I obligate methylotroph, Methylococcus capsulatus.

作者信息

Patel R N, Hoare S L, Hoare D S, Taylor B F

出版信息

Appl Environ Microbiol. 1977 Nov;34(5):607-10. doi: 10.1128/aem.34.5.607-610.1977.

Abstract

Methanol and formate oxidation supported the assimilation of [14C]acetate by cell suspensions of Methylococcus capsulatus; oxidation of other primary alcohols, except ethanol, did not. The extent of [1-14C]acetate assimilation supported by methanol oxidation was decreased in the presence of primary alcohols, except ethanol. Potassium cyanide (0.33 mM) completely inhibited the oxidation of formate and its stimulation of [1-14C]acetate assimilation. The amount of [1-14C]acetate assimilation supported by methanol oxidation was significantly inhibited by cyanide.

摘要

甲醇和甲酸盐氧化支持荚膜甲基球菌细胞悬浮液对[14C]乙酸盐的同化作用;除乙醇外,其他伯醇的氧化则不能。在存在除乙醇外的伯醇时,由甲醇氧化支持的[1-14C]乙酸盐同化程度降低。氰化钾(0.33 mM)完全抑制甲酸盐的氧化及其对[1-14C]乙酸盐同化的刺激作用。氰化物显著抑制由甲醇氧化支持的[1-14C]乙酸盐同化量。

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