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火鸡支原体的超微结构与荚膜

Ultrastructure and capsule of Mycoplasma meleagridis.

作者信息

Green F, Hanson R P

出版信息

J Bacteriol. 1973 Nov;116(2):1011-8. doi: 10.1128/jb.116.2.1011-1018.1973.

Abstract

The ultrastructural study of Mycoplasma meleagridis utilized the electron microscope techniques of sectioning, histochemical staining, critical-point drying, and freeze etching. The predominant morphotype was a spherical form ranging in diameter from 200 to 700 nm. The other morphotypes were dumbbell-shaped cells interconnected by membranous tubules, and chains of streptococcal-like cells. These forms suggest replication by binary fission. An extracellular structure in the form of a capsular matrix was observed by staining with ruthenium red and potassium tellurite, and was also seen in specimens prepared by critical-point drying and freeze etching.

摘要

利用切片、组织化学染色、临界点干燥和冷冻蚀刻等电子显微镜技术对火鸡支原体进行了超微结构研究。主要的形态类型是球形,直径在200至700纳米之间。其他形态类型是通过膜性小管相互连接的哑铃形细胞,以及链状的类链球菌细胞。这些形态表明通过二分裂进行繁殖。通过用钌红和亚碲酸钾染色观察到一种呈荚膜基质形式的细胞外结构,在临界点干燥和冷冻蚀刻制备的标本中也可见到。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9db9/285476/4911a81997d4/jbacter00578-0517-a.jpg

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