脲原体中脂聚糖的检测。
Detection of lipoglycans in ureaplasmas.
作者信息
Smith P F
出版信息
J Bacteriol. 1985 May;162(2):611-4. doi: 10.1128/jb.162.2.611-614.1985.
Abstract
Serotypes 3, 4, and 8 of Ureaplasma urealyticum were found to contain lipoglycans. Although the ratios of their components differed, all contained neutral sugars, fatty acids, glycerol, and phosphorus. All three became labeled when the organisms were cultivated in the presence of [14C]glucose, [14C]palmitic or [14C]oleic acids, and inorganic 32P. Only neutral sugars were found, and these consisted of mannose, glucose, and galactose. Hot phenol extracts of uninoculated and supernatant culture media contained polymeric carbohydrate, but this differed in composition from ureaplasmal lipoglycans and did not become radiolabeled. Since lipoglycans contained phosphorus but no amino sugars, they could be separated from contaminating polysaccharides by anion exchange chromatography.
摘要
解脲脲原体的血清型3、4和8被发现含有脂聚糖。尽管它们各成分的比例不同，但都含有中性糖、脂肪酸、甘油和磷。当这些微生物在[14C]葡萄糖、[14C]棕榈酸或[14C]油酸以及无机32P存在的情况下培养时，所有三种血清型都会被标记。只发现了中性糖，这些中性糖由甘露糖、葡萄糖和半乳糖组成。未接种的和上清培养基的热酚提取物含有聚合碳水化合物，但这种聚合碳水化合物的组成与脲原体脂聚糖不同，并且不会被放射性标记。由于脂聚糖含有磷但不含氨基糖，因此可以通过阴离子交换色谱法将它们与污染的多糖分离。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Endotoxin-like activities of mycoplasmal lipopolysaccharides (lipoglycans).支原体脂多糖（脂聚糖）的类内毒素活性
Infect Immun. 1980 Sep;29(3):990-4. doi: 10.1128/iai.29.3.990-994.1980.
3
Sequence and glycosidic bond arrangement of sugars in lipopolysaccharide from Thermoplasma acidophilum.嗜热栖热菌脂多糖中糖的序列和糖苷键排列
Biochim Biophys Acta. 1980 Aug 11;619(2):367-73. doi: 10.1016/0005-2760(80)90084-3.
4
Structure of the oligosaccharide chain of lipoglycan from Acholeplasma granularum.颗粒无胆甾原体脂聚糖寡糖链的结构
Biochim Biophys Acta. 1981 Jul 24;665(1):92-9. doi: 10.1016/0005-2760(81)90237-x.
5
Humoral immune response of rabbits to acholeplasmal lipoglycans.兔子对无胆甾原体脂多糖的体液免疫反应。
Infect Immun. 1980 Sep;29(3):926-33. doi: 10.1128/iai.29.3.926-933.1980.
6
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Infect Immun. 1983 May;40(2):629-32. doi: 10.1128/iai.40.2.629-632.1983.
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Isolation and characterization of the sheep erythrocyte receptor for acholeplasmal lipoglycans.无胆甾原体脂多糖绵羊红细胞受体的分离与鉴定
Infect Immun. 1982 Dec;38(3):1078-87. doi: 10.1128/iai.38.3.1078-1087.1982.
8
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Biochem J. 1964 Jan;90(1):201-5. doi: 10.1042/bj0900201.
9
Enhanced growth of T-strain mycoplasmas with N-2-hydroxyethylpiperazone-N'-2-ethanesulfonic acid buffer.用N-2-羟乙基哌嗪-N'-2-乙磺酸缓冲液促进T株支原体生长。
J Bacteriol. 1969 Oct;100(1):78-85. doi: 10.1128/jb.100.1.78-85.1969.
10
Heptose-containing pentaglycosyl diglyceride among the lipids of Acholeplasma modicum.微小无胆甾原体脂质中的含庚糖五糖苷二甘油酯。
J Bacteriol. 1974 Jun;118(3):898-904. doi: 10.1128/jb.118.3.898-904.1974.
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