铜绿假单胞菌中的肽利用：膜相关肽酶的证据。
Peptide utilization in Pseudomonas aeruginosa: evidence for membrane-associated peptidase.
作者信息
Miller R V, Becker J M
出版信息
J Bacteriol. 1978 Jan;133(1):165-71. doi: 10.1128/jb.133.1.165-171.1978.
Abstract
A methionine auxotroph of Pseudomonas aeruginosa grew on methionine-containing peptides as a source of the required amino acid. Amino-terminus-blocked peptides would not serve as growth substrates, despite the fact that peptidases active on these blocked peptides were readily detectable in cell extracts. No evidence was found for extracellular enzymes capable of degrading the oligopeptides investigated. The degradative enzymes were not found in the periplasmic space of the cellular envelope. A high proportion of cellular peptidase activity was associated with the particulate (membrane) fraction of the cell lysate.
摘要
铜绿假单胞菌的一种甲硫氨酸营养缺陷型菌株能够利用含甲硫氨酸的肽作为所需氨基酸的来源进行生长。尽管在细胞提取物中很容易检测到对这些N端封闭肽有活性的肽酶，但N端封闭的肽不能作为生长底物。未发现有能够降解所研究寡肽的细胞外酶。在细胞被膜的周质空间中未发现降解酶。细胞裂解物的颗粒（膜）部分与细胞内大部分肽酶活性相关。
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