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由豇豆花叶病毒蛋白包裹的小核糖核酸病毒RNA的结构与感染性

Structure and infectivity of picornaviral RNA encapsidated by cowpea chlorotic mottle virus protein.

作者信息

Black D R, Connell C J, Merigan T C

出版信息

J Virol. 1973 Dec;12(6):1209-15. doi: 10.1128/JVI.12.6.1209-1215.1973.

DOI:10.1128/JVI.12.6.1209-1215.1973
PMID:4128376
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC356761/
Abstract

Poliovirus and Mengo virus RNA were shown to associate efficiently with cowpea chlorotic mottle virus protein to form pseudovirions. The sedimentation coefficient for the pseudovirions was similar to that of poliovirus, and electron microscope observations showed the Mengo pseudovirions to be similar in size to Mengo virus. Such pseudovirions were infectious and were more resistant to ribonuclease than viral RNA; however, under our assay conditions, their infectivity was about equal to that of viral RNA.

摘要

脊髓灰质炎病毒和门戈病毒的RNA能有效地与豇豆褪绿斑驳病毒蛋白结合,形成假病毒颗粒。假病毒颗粒的沉降系数与脊髓灰质炎病毒相似,电子显微镜观察显示门戈假病毒颗粒的大小与门戈病毒相似。这种假病毒颗粒具有感染性,并且比病毒RNA对核糖核酸酶更具抗性;然而,在我们的检测条件下,它们的感染性与病毒RNA大致相当。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf09/356761/8a5ee28ca568/jvirol00264-0035-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf09/356761/8a5ee28ca568/jvirol00264-0035-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf09/356761/8a5ee28ca568/jvirol00264-0035-a.jpg

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Structure and infectivity of picornaviral RNA encapsidated by cowpea chlorotic mottle virus protein.由豇豆花叶病毒蛋白包裹的小核糖核酸病毒RNA的结构与感染性
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本文引用的文献

1
RECONSTITUTION OF ACTIVE TOBACCO MOSAIC VIRUS FROM ITS INACTIVE PROTEIN AND NUCLEIC ACID COMPONENTS.由非活性蛋白和核酸成分重建活性烟草花叶病毒
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Plaque formation and isolation of pure lines with poliomyelitis viruses.
脊髓灰质炎病毒的噬斑形成及纯系分离
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Properties of cowpea chlorotic mottle virus, its protein and nucleic acid.豇豆褪绿斑驳病毒及其蛋白质和核酸的特性。
Virology. 1968 Feb;34(2):224-39. doi: 10.1016/0042-6822(68)90232-8.
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Reconstitution of viruses.病毒的重组
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Structure and assembly of simple RNA bacteriophages.简单RNA噬菌体的结构与组装
Adv Virus Res. 1970;16:43-98. doi: 10.1016/s0065-3527(08)60021-4.