鸡淋巴细胞的抗原表面决定簇。I. 抗腔上囊血清和抗胸腺血清的血清学特性。
Antigenic surface determinants of chicken lymphoid cells. I. Serologic properties of anti-bursa and anti-thymus sera.
作者信息
Wick G, Albini B, Milgrom F
出版信息
Clin Exp Immunol. 1973 Oct;15(2):237-49.
The serologic properties of turkey antisera to chicken bursa (ABS) and thymus (ATS) cells were assayed by cytolysis-in-agar, conventional cytotoxicity tests and indirect immunofluorescence. Using proper absorptions the following antigenic surface determinants were detected on bursal and thymic lymphoid cells: (a) common lymphocyte antigens present on both kinds of cells; (b) thymus-specific antigens; (c) bursa-specific antigens; (d) in addition to the latter, bursa cells displayed immunoglobulin surface determinants. Even before absorption the anti-thymus and anti-bursa sera gave higher titres of reactions with homologous target cell preparations. In complement dependent cytotoxicity tests ABS and ATS reacted specifically with bursa and thymus cells respectively. In the spleen of 2- and 3-week-old chickens 20–30% of lymphoid cells were killed by ABS and 40–50% by ATS. The advantage of avian antilymphocyte sera for studies in chickens are emphasized.
采用琼脂中的细胞溶解法、传统细胞毒性试验和间接免疫荧光法检测了火鸡抗鸡法氏囊（ABS）和胸腺（ATS）细胞血清的血清学特性。通过适当的吸收处理，在法氏囊和胸腺淋巴样细胞上检测到了以下抗原表面决定簇：（a）两种细胞上都存在的共同淋巴细胞抗原；（b）胸腺特异性抗原；（c）法氏囊特异性抗原；（d）除后者外，法氏囊细胞还显示出免疫球蛋白表面决定簇。甚至在吸收处理之前，抗胸腺血清和抗法氏囊血清与同源靶细胞制剂的反应滴度就更高。在补体依赖的细胞毒性试验中，ABS和ATS分别与法氏囊和胸腺细胞发生特异性反应。在2周龄和3周龄鸡的脾脏中，20% - 30%的淋巴样细胞被ABS杀死，40% - 50%被ATS杀死。强调了禽抗淋巴细胞血清在鸡研究中的优势。
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