球形节杆菌噬菌体的分离与鉴定

Isolation and characterization of a bacteriophage of Arthrobacter globiformis.

作者信息

Einck K H, Pattee P A, Holt J G, Hagedorn C, Miller J A, Berryhill D L

出版信息

J Virol. 1973 Nov;12(5):1031-3. doi: 10.1128/JVI.12.5.1031-1033.1973.

DOI:10.1128/JVI.12.5.1031-1033.1973

PMID:4128824

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC356733/

Abstract

A bacteriophage which reproduces on Arthrobacter globiformis ATCC 8010 was isolated from soil. This bacteriophage, designated phiAG8010, propagates either in soft agar or broth cultures of the host. Because of a slow adsorption rate, neither the latent period nor burst size was determined. The mature virion belongs to Bradley's group B and exhibits a hexagonal head measuring 69 nm (length) by 60 nm (width) attached to a sheathless tail 120 nm long. The buoyant density of the mature virion is 1.534 g/cm(3). The mature virion contains double-stranded DNA with a buoyant density of 1.722 g/cm(3) (equivalent to 63.3% G + C). Of 14 strains (representing 13 species) of Arthrobacter examined, including A. globiformis ATCC 4336, only A. globiformis ATCC 8010 supported replication of phiAG8010.

摘要

从土壤中分离出一种能在球形节杆菌ATCC 8010上繁殖的噬菌体。这种噬菌体被命名为phiAG8010，可在宿主的软琼脂或肉汤培养物中繁殖。由于吸附速率较慢，因此未测定潜伏期和裂解量。成熟的病毒粒子属于布拉德利B组，呈现出一个六边形头部，长69纳米（长），宽60纳米（宽），连接着一条120纳米长的无鞘尾部。成熟病毒粒子的浮力密度为1.534克/立方厘米。成熟病毒粒子含有双链DNA，浮力密度为1.722克/立方厘米（相当于63.3%的鸟嘌呤+胞嘧啶）。在检测的14株节杆菌（代表13个物种）中，包括球形节杆菌ATCC 4336，只有球形节杆菌ATCC 8010支持phiAG8010的复制。