Trautwetter A, Blanco C, Bonnassie S
Laboratoire de Microbiologie, INSA, Villeurbanne, France.
J Gen Microbiol. 1987 Oct;133(10):2945-52. doi: 10.1099/00221287-133-10-2945.
Bacteriophage CG33 was isolated from a strain of Corynebacterium glutamicum that had become contaminated during an industrial fermentation. CG33 was assigned to Bradley's group B since it had a polyhedral head 40 nm wide and a short non-contractile and striated tail 78 nm long. Adsorption to its host, C. glutamicum ATCC 13287, was enhanced in the presence of Ca2+. The latent period was 18 min at 34 degrees C; the burst size was 16 p.f.u. ml-1. CG33 also formed plaques on C. lilium ATCC 15990 but at a low frequency. Its genome consisted of a linear double stranded DNA molecule of 13.4 kb with cohesive ends. A restriction map of the genome was obtained by using various endonucleases.
噬菌体CG33是从一株在工业发酵过程中被污染的谷氨酸棒杆菌中分离出来的。由于CG33具有一个40纳米宽的多面体头部和一条78纳米长的短的非收缩性横纹尾部，因此被归入布拉德利的B组。在Ca2+存在的情况下，它对宿主谷氨酸棒杆菌ATCC 13287的吸附作用增强。在34摄氏度时潜伏期为18分钟；裂解量为每毫升16个噬菌斑形成单位。CG33在百合棒杆菌ATCC 15990上也能形成噬菌斑，但频率较低。其基因组由一个13.4千碱基对的线性双链DNA分子组成，两端具有粘性末端。通过使用各种核酸内切酶获得了该基因组的限制性图谱。
