Klein F, de Bruyn A M, Radema H
Clin Exp Immunol. 1973 Sep;15(1):103-11.
By means of the single radial immunodiffusion technique it is possible to detect at least two subgroups of human IgM paraproteins, if the square ring diameter is plotted against absolute concentration. These groups give different calibration lines with the same antiserum, even when no qualitative differences can be detected by the double diffusion technique. The possibility of distinguishing these groups is not limited to the use of a single antiserum. The differences between the groups are abolished by reduction of the IgM paraprotein to 7S subunits and therefore seem to be of conformational nature. No identity with other known classifications has yet been found. In one out of two cases investigated differences in quantitative reactivity were found between 19S and >19S components in the same serum. The results suggest that quantitative methods may be used to detect differences in immunoglobulin structure, when qualitative methods fail. They show that different human IgM paraproteins cannot be directly compared quantitatively by the radial immunodiffusion method.
通过单放射免疫扩散技术，如果以方环直径对绝对浓度作图，就有可能检测出人类IgM副蛋白的至少两个亚组。即使双向扩散技术检测不出定性差异，这些亚组用同一种抗血清也会给出不同的校准曲线。区分这些亚组的可能性并不局限于使用单一抗血清。通过将IgM副蛋白还原为7S亚基，这些亚组之间的差异就消失了，因此似乎是构象性质的。尚未发现与其他已知分类有一致性。在所研究的两个病例中的一个，发现同一血清中19S和>19S成分之间存在定量反应性差异。结果表明，当定性方法失效时，定量方法可用于检测免疫球蛋白结构的差异。结果表明，不同的人类IgM副蛋白不能通过放射免疫扩散法直接进行定量比较。
