Anneroth G, Eneroth C M, Isacsson G
J Oral Pathol. 1977 Nov;6(6):373-81. doi: 10.1111/j.1600-0714.1977.tb01803.x.
Using lipid histochemical and microradiographic methods, the distribution of lipids and the inorganic component in different areas of salivary calculi was investigated. Two main groups of lipids were found, i.e. hydrophilic phospholipids and hydrophobic lipids. The hydrophilic phospholipids were shown to be composed mainly of phosphosphingosides and of the hydrophobic lipids cholesterol was demonstrated. Phosphosphingosides were localized in more or less lamellar zones as also in large homogeneous areas. In some cases the calculi were surrounded by a structureless non-mineralized zone exhibiting a strongly positive reaction for phosphosphingosides. Cholesterol was found in isolated band-formed areas. Phosphosphingosides and cholesterol were especially prevalent in areas showing a low or slight degree of mineralization. In areas showing a high degree of mineralization the corresponding lipids gave a weak or absent histochemical reaction. After EDTA-decalcification, however, an increase in the concentration of lipid was noticed in high mineralized areas, indicating a masking process. The influence of various factors on the presence and distribution of different lipids was discussed.
采用脂质组织化学和显微放射照相方法，研究了唾液结石不同区域脂质和无机成分的分布。发现了两大类脂质，即亲水性磷脂和疏水性脂质。亲水性磷脂主要由磷酸鞘氨醇组成，疏水性脂质则检测出胆固醇。磷酸鞘氨醇定位于或多或少的层状区域以及大的均质区域。在某些情况下，结石被无结构的非矿化区域包围，该区域对磷酸鞘氨醇呈现强烈阳性反应。胆固醇存在于孤立的带状区域。磷酸鞘氨醇和胆固醇在矿化程度低或轻微的区域尤其普遍。在矿化程度高的区域，相应的脂质组织化学反应较弱或无反应。然而，经乙二胺四乙酸脱钙后，在高矿化区域观察到脂质浓度增加，表明存在掩盖过程。讨论了各种因素对不同脂质存在和分布的影响。
