Bâlici Ștefana, Florea Adrian, Al-Hajaj Ameen Ibrahim, Chiorean Alin-Dan, Nicula Gheorghe Zsolt
Department of Cell and Molecular Biology, Faculty of Medicine, "Iuliu Hațieganu" University of Medicine and Pharmacy, 6 Louis Pasteur St., 400349 Cluj-Napoca, Romania.
Molecules. 2025 Nov 15;30(22):4419. doi: 10.3390/molecules30224419.
Bee venom (BV) molecules, including melittin (Mlt), are known to modify the permeability of membranes. This paper assessed red blood cell (RBC) shape (by phase contrast microscopy) in relation to some of the parameters (haematology data) and calculated the RBC membranes' water diffusional permeability (P) with H-NMR spectroscopy. Rats were injected for 30 days with either small daily doses of BV (VST) or Mlt (MST) or with high single doses of BV (VSLT) or Mlt (MSLT). The RBCs displayed aberrant shapes, all of the analysed parameters significantly changed, and the values of P were higher (and increased with temperature) in all of the treated groups compared to the control group. The RBCs in the venom-treated groups had the highest mean values (expressed in cm × s × 10) of P at 37 °C-8.95 in the VSLT group and 8.69 in the VST group-which were followed by the MST and MSLT groups and the control group. Our results demonstrated the ability of Mlt to retain the ability to interact with the RBC membrane in vivo and proved that Mlt is the most important BV molecule involved in this process.
已知包括蜂毒肽（Mlt）在内的蜂毒（BV）分子会改变膜的通透性。本文通过相差显微镜评估了红细胞（RBC）形态与一些参数（血液学数据）的关系，并利用氢核磁共振波谱法计算了红细胞膜的水扩散通透性（P）。给大鼠连续注射30天，分别给予每日小剂量的BV（VST）或Mlt（MST），或单次大剂量的BV（VSLT）或Mlt（MSLT）。红细胞呈现异常形态，所有分析参数均发生显著变化，与对照组相比，所有处理组的P值均更高（且随温度升高而增加）。在37℃时，毒液处理组红细胞的P平均值（以厘米×秒×10表示）最高——VSLT组为8.95，VST组为8.69，其次是MST组、MSLT组和对照组。我们的结果证明了Mlt在体内保持与红细胞膜相互作用的能力，并证明Mlt是参与这一过程的最重要的BV分子。
